Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Werner, ER; Werner-Felmayer, G; Fuchs, D; Hausen, A; Reibnegger, G; Yim, JJ; Wachter, H.
Impact of tumour necrosis factor-alpha and interferon-gamma on tetrahydrobiopterin synthesis in murine fibroblasts and macrophages.
Biochem J. 1991; 280 ( Pt 3)(3):709-714
Doi: 10.1042/bj2800709
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- Co-Autor*innen der Med Uni Graz
- Reibnegger Gilbert
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- Abstract:
- Tumour necrosis factor-alpha causes an up to 30-fold induction of GTP cyclohydrolase I (EC 3.5.4.16) activity in murine dermal fibroblasts in a dose-dependent manner. Owing to the high constitutive activities of 6-pyruvoyltetrahydropterin synthase and sepiapterin reductase (EC 1.1.1.153), this potentiates biosynthesis of tetrahydrobiopterin. Murine macrophages already contain high activities of GTP cyclohydrolase I when unstimulated, and this is further augmented up to 4-fold by tumour necrosis factor-alpha/interferon-gamma. In Western blots an antiserum to murine liver GTP cyclohydrolase I does not stain cell extracts with high enzyme activities, suggesting that the cytokine induced peripheral form of GTP cyclohydrolase I might differ from the liver form.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Biopterin - analogs and derivatives
- Cells, Cultured - analogs and derivatives
- Cycloheximide - pharmacology
- Fibroblasts - drug effects
- GTP Cyclohydrolase - metabolism
- Interferon-gamma, Recombinant - pharmacology
- Liver - enzymology
- Macrophages - drug effects
- Mice - drug effects
- Mice, Inbred BALB C - drug effects
- Tumor Necrosis Factor-alpha - pharmacology