Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

Direkt zur Navigaton springen.
Direkt zum Inhalt springen.

Navigation/Suche

Gewählte Publikation:

Werner-Felmayer, G; Werner, ER; Fuchs, D; Hausen, A; Mayer, B; Reibnegger, G; Weiss, G; Wachter, H.
Ca2+/calmodulin-dependent nitric oxide synthase activity in the human cervix carcinoma cell line ME-180.
Biochem J. 1993; 289 ( Pt 2)(2):357-361 Doi: 10.1042/bj2890357 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG Google Scholar

Co-Autor*innen der Med Uni Graz: Reibnegger Gilbert
Altmetrics:
Dimensions Citations:
Plum Analytics:
Scite (citation analytics):
Abstract:: We show here that the human cervix carcinoma cell line ME-180 expresses a constitutive nitric oxide (NO) synthase, as demonstrated by formation of [3H]citrulline and nitrite. The enzyme is dependent on tetrahydrobiopterin, NADPH, flavins and Ca2+/calmodulin. Enzyme activity is located in the cytosol rather than in the membrane fraction and can be inhibited by NG-monomethyl-L-arginine (NMMA). An antiserum to NO synthase purified from porcine cerebellum inhibited the enzyme activity. ME-180 cells released NO, as was shown by stimulation of guanylate cyclase (EC 4.6.1.2) in RFL-6 detector cells; this release was stimulated 8-fold by the Ca2+ ionophore A23187 and 2-fold by increasing the intracellular tetrahydrobiopterin levels with cytokines. This is the first characterization of a Ca2+/calmodulin-dependent NO synthase activity in human epithelial-type tumour cells.
Find related publications in this database (using NLM MeSH Indexing): Amino Acid Oxidoreductases - metabolism; Arginine - analogs and derivatives; Calcium - pharmacology; Calmodulin - pharmacology; Cyclic GMP - metabolism; Female - metabolism; Humans - metabolism; Interferon-gamma, Recombinant - pharmacology; Kinetics - pharmacology; Lipopolysaccharides - pharmacology; Nitric Oxide Synthase - pharmacology; Pteridines - pharmacology; Tumor Cells, Cultured - pharmacology; Uterine Cervical Neoplasms - enzymology; omega-N-Methylarginine - enzymology