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SHR Neuro Cancer Cardio Lipid Metab Microb

Schwaminger, SP; Blank-Shim, SA; Scheifele, I; Pipich, V; Fraga-García, P; Berensmeier, S.
Design of Interactions Between Nanomaterials and Proteins: A Highly Affine Peptide Tag to Bare Iron Oxide Nanoparticles for Magnetic Protein Separation.
Biotechnol J. 2019; 14(3):e1800055 Doi: 10.1002/biot.201800055
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Leading authors Med Uni Graz: Schwaminger Sebastian
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Abstract:: Superparamagnetic nanoparticles have recently gained much attention due to their broad range of applicability including medical in vivo technologies, sensors, and as supports for catalysts. As magnetic affinity materials, they can be utilized for the development of new purification strategies for pharmaceuticals and other target molecules from crude lysates. Here, a short peptide tag based on a glutamate sequence is introduced and the adsorption of pure protein as well as protein from crude cell lysate at different conditions is demonstrated. Fused to a model protein this tag can be used to recognize and purify this protein from a fermentation broth by bare iron oxide nanoparticles (BIONs). Binding of up to 0.2 g protein per g nanoparticles can be achieved and recovered easily by switching to a citrate buffered system. For a deeper understanding of the separation process, the aggregation and agglomeration of the nanoparticle protein systems were monitored for binding and elution steps. Furthermore, an upscaling of the process to the liter scale and the separation of a green fluorescent protein (GFP) containing the affinity tag to purities of 70% from Escherichia coli fermentation broth was possible in a one step process by means of high gradient magnetic separation (HGMS).
Find related publications in this database (using NLM MeSH Indexing): Adsorption - administration & dosage; Chromatography, Affinity - methods; Escherichia coli - metabolism; Ferric Compounds - chemistry; Green Fluorescent Proteins - chemistry; Magnetics - methods; Magnetite Nanoparticles - chemistry; Oligopeptides - chemistry; Peptides - chemistry; Proteins - chemistry
Find related publications in this database (Keywords): affinity tags; iron oxides; magnetic nanoparticles; protein purification; separation process