Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Ghini, V; Abuja, PM; Polasek, O; Kozera, L; Laiho, P; Anton, G; Zins, M; Klovins, J; Metspalu, A; Wichmann, HE; Gieger, C; Luchinat, C; Zatloukal, K; Turano, P.
Impact of the pre-examination phase on multicenter metabolomic studies.
N Biotechnol. 2022; 68:37-47
Doi: 10.1016/j.nbt.2022.01.006
Web of Science
PubMed
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FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Zatloukal Kurt
- Co-Autor*innen der Med Uni Graz
- Abuja Peter Michael
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- Abstract:
- The development of metabolomics in clinical applications has been limited by the lack of validation in large multicenter studies. Large population cohorts and their biobanks are a valuable resource for acquiring insights into molecular disease mechanisms. Nevertheless, most of their collections are not tailored for metabolomics and have been created without specific attention to the pre-analytical requirements for high-quality metabolome assessment. Thus, comparing samples obtained by different pre-analytical procedures remains a major challenge. Here, 1H NMR-based analyses are used to demonstrate how human serum and plasma samples collected with different operating procedures within several large European cohort studies from the Biobanking and Biomolecular Resources Infrastructure - Large Prospective Cohorts (BBMRI-LPC) consortium can be easily revealed by supervised multivariate statistical analyses at the initial stages of the process, to avoid biases in the downstream analysis. The inter-biobank differences are discussed in terms of deviations from the validated CEN/TS 16945:2016 / ISO 23118:2021 norms. It clearly emerges that biobanks must adhere to the evidence-based guidelines in order to support wider-scale application of metabolomics in biomedicine, and that NMR spectroscopy is informative in comparing the quality of different sample sources in multi cohort/center studies.
- Find related publications in this database (Keywords)
- Metabolomics
- Pre-analytical procedures
- NMR
- Biobanks