Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Kroisel, PM; Ioannou, PA; de Jong, PJ.
PCR probes for chromosome in situ hybridization of large-insert bacterial recombinants.
CYTOGENET CELL GENET. 1994; 65(1-2): 97-100.
Doi: 10.1159/000133609
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PubMed
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- Führende Autor*innen der Med Uni Graz
- Kroisel Peter
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- Abstract:
- We have developed a procedure for efficient in situ hybridization of bacterial recombinants created with various types of large-insert cloning vectors. Minimal quantities of crude DNA are amplified and labeled during the degenerate-oligonucleotide-primed polymerase chain reaction. The resulting probes generate high-intensity fluorescent hybridization signals on metaphase chromosomes and on interphase nuclei.
- Find related publications in this database (using NLM MeSH Indexing)
- Cloning, Molecular -
- DNA Probes -
- DNA, Bacterial - analysis
- DNA, Recombinant - analysis
- Genetic Vectors - analysis
- In Situ Hybridization, Fluorescence - analysis
- Polymerase Chain Reaction - methods