Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Koterniak, B; Pilaka, PP; Gracida, X; Schneider, LM; Pritišanac, I; Zhang, Y; Calarco, JA.
Global regulatory features of alternative splicing across tissues and within the nervous system of C. elegans.
Genome Res. 2020; 30(12):1766-1780
Doi: 10.1101/gr.267328.120
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- Co-Autor*innen der Med Uni Graz
- Pritisanac Iva
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- Abstract:
- Alternative splicing plays a major role in shaping tissue-specific transcriptomes. Among the broad tissue types present in metazoans, the central nervous system contains some of the highest levels of alternative splicing. Although many documented examples of splicing differences between broad tissue types exist, there remains much to be understood about the splicing factors and the cis sequence elements controlling tissue and neuron subtype-specific splicing patterns. By using translating ribosome affinity purification coupled with deep-sequencing (TRAP-seq) in Caenorhabditis elegans, we have obtained high coverage profiles of ribosome-associated mRNA for three broad tissue classes (nervous system, muscle, and intestine) and two neuronal subtypes (dopaminergic and serotonergic neurons). We have identified hundreds of splice junctions that exhibit distinct splicing patterns between tissue types or within the nervous system. Alternative splicing events differentially regulated between tissues are more often frame-preserving, are more highly conserved across Caenorhabditis species, and are enriched in specific cis regulatory motifs, when compared with other types of exons. By using this information, we have identified a likely mechanism of splicing repression by the RNA-binding protein UNC-75/CELF via interactions with cis elements that overlap a 5' splice site. Alternatively spliced exons also overlap more frequently with intrinsically disordered peptide regions than constitutive exons. Moreover, regulated exons are often shorter than constitutive exons but are flanked by longer intron sequences. Among these tissue-regulated exons are several highly conserved microexons <27 nt in length. Collectively, our results indicate a rich layer of tissue-specific gene regulation at the level of alternative splicing in C. elegans that parallels the evolutionary forces and constraints observed across metazoa.
- Find related publications in this database (using NLM MeSH Indexing)
- Alternative Splicing - administration & dosage
- Animals - administration & dosage
- Caenorhabditis elegans - genetics, metabolism
- Caenorhabditis elegans Proteins - genetics, metabolism
- Exons - administration & dosage
- Gene Expression Profiling - methods
- Gene Expression Regulation - administration & dosage
- High-Throughput Nucleotide Sequencing - administration & dosage
- Intestines - chemistry
- Muscle, Skeletal - chemistry
- Nervous System - chemistry
- Organ Specificity - administration & dosage
- RNA-Binding Proteins - metabolism
- Sequence Analysis, RNA - administration & dosage
- Tissue Distribution - administration & dosage