Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Narimisa, N; Amraei, F; Kalani, BS; Mohammadzadeh, R; Jazi, FM.
Effects of sub-inhibitory concentrations of antibiotics and oxidative stress on the expression of type II toxin-antitoxin system genes in Klebsiella pneumoniae.
J Glob Antimicrob Resist. 2020; 21: 51-56.
Doi: 10.1016/j.jgar.2019.09.005
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Mohammadzadeh Rokhsareh
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- Abstract:
- OBJECTIVES: Sub-inhibitory concentrations (sub-MICs) of antibiotics reflect the conditions that bacteria encounter in tissues and the natural environment. Sub-MICs of antibiotics can induce stress and alter the expression of different bacterial genes. Bacteria react to stress conditions using different mechanisms, one of which is the toxin-antitoxin (TA) system. This study investigated the expression of the TA system genes under oxidative and antibiotic stresses in Klebsiella pneumoniae (K. pneumoniae). METHODS: To determine the effects of sub-MICs of gentamicin, nalidixic acid, ceftazidime, and certain concentrations of H2O2 on bacterial survival and growth, colony forming units were quantitated and turbidity was assessed following the treatment of K. pneumoniae with ½ MICs of antibiotics and 5 mM H2O2 at different time intervals. The expression of TA system genes in K. pneumoniae was evaluated 1 h after treatment using the quantitative real-time PCR (qRT-PCR) method. RESULTS: The results revealed reduced K. pneumoniae growth in the presence of sub-MICs of antibiotics and 5 mM H2O2 compared to the control. Furthermore, according to the results of the qRT-PCR assay, only the presence of gentamicin could increase the expression of TA system genes. CONCLUSION: Although the exact role of the TA systems in response to stress is still unclear, this study provided information on the effect of the type II TA systems under oxidative and antibiotic stress conditions.
- Find related publications in this database (using NLM MeSH Indexing)
- Anti-Bacterial Agents - pharmacology
- Bacterial Proteins - genetics
- Ceftazidime - pharmacology
- Gene Expression Regulation, Bacterial - drug effects
- Gentamicins - pharmacology
- Hydrogen Peroxide - pharmacology
- Klebsiella pneumoniae - drug effects, genetics, growth & development
- Microbial Sensitivity Tests - administration & dosage
- Microbial Viability - drug effects
- Nalidixic Acid - pharmacology
- Oxidative Stress - administration & dosage
- Toxin-Antitoxin Systems - drug effects
- Find related publications in this database (Keywords)
- Sub-inhibitory antibiotic concentrations
- Oxidative stress
- Klebsiella pneumonia
- Toxin-antitoxin systems
- Real-time PCR