Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Shivaee, A; Mohammadzadeh, R; Shahbazi, S; Pardakhtchi, E; Ohadi, E; Kalani, BS.
Time-variable expression levels of mazF, atlE, sdrH, and bap genes during biofilm formation in Staphylococcus epidermidis.
Acta Microbiol Immunol Hung. 2019; 66(4): 499-508.
Doi: 10.1556/030.66.2019.019
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Mohammadzadeh Rokhsareh
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- Abstract:
- Staphylococcus epidermidis is an opportunistic pathogen causing infections related to the usage of implants and medical devices. Pathogenicity of this microorganism is mainly linked to its capability to form biofilm structures. Biofilm formation vastly depends on several factors including different proteins. We studied the expression levels of three proteins including SdrH, Bap, AtlE, and MazF at different time intervals during the course of biofilm formation. In this study, a catheter-derived S. epidermidis isolate with strong ability of biofilm formation was selected. PCR assay was used to detect sdrH, bap, atlE, and mazF genes in this isolate. Real-time PCR was used to determine the expression levels of these genes after 4, 8, and 20 h during the course of biofilm formation. The studied genes showed different expression levels at different time intervals during biofilm formation by real-time PCR method. Expression levels of atlE and sdrH genes were the highest at 4 h, whereas bap gene showed the highest expression level at 8 h during the course of biofilm formation. In addition, the expression level of mazF gene peaked at 4 h and then progressively decreased at 8 and 20 h. Our results suggest the importance of AtlE, SdrH, and MazF proteins in the establishment and development of the biofilm structure. In addition, our results showed the important role of protein Bap in the accumulation of biofilm structure. Future studies are required to understand the exact role of MazF in the process of biofilm formation.
- Find related publications in this database (using NLM MeSH Indexing)
- Bacterial Adhesion - administration & dosage
- Bacterial Proteins - genetics
- Biofilms - growth & development
- Gene Expression Regulation, Bacterial - administration & dosage
- Staphylococcus epidermidis - genetics, physiology
- Time Factors - administration & dosage
- Find related publications in this database (Keywords)
- Staphylococcus epidermidis
- biofilm formation
- toxin/antitoxin systems
- real-time PCR