Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Javanmard, Z; Kalani, BS; Razavi, S; Farahani, NN; Mohammadzadeh, R; Javanmard, F; Irajian, G.
Evaluation of cell-penetrating peptide-peptide nucleic acid effect in the inhibition of cagA in Helicobacter pylori.
Acta Microbiol Immunol Hung. 2020; 67(1): 66-72.
Doi: 10.1556/030.66.2019.032
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Mohammadzadeh Rokhsareh
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- Abstract:
- Helicobacter pylori is the most common cause of chronic infection in human and is associated with gastritis, peptic ulcer disease, and adenocarcinoma of mucosa-associated lymphoid tissue cells. Peptide nucleic acid (PNA) is a synthetic compound, which can inhibit the production of a particular gene. This study aimed to investigate the effect of PNA on inhibiting the expression of cagA. After confirmation of the desired gene by polymerase chain reaction (PCR), the antisense sequence was designed against cagA gene. The minimum inhibitory concentrations of conjugated PNA against H. pylori was determined. The effect of the compound on the expression level of the cagA was investigated in HT29 cell culture using real-time PCR. The results showed 2 and 3 log reduction in bacterial count after 8- and 24-h treatment with 4 and 8 μM of the compound, respectively. The lowest expression level of the cagA gene was observed at a concentration of 8 μM after 6 h. The results of this study showed that cell-penetrating peptide antisense can be employed as effective tools for inhibiting the target gene mRNA for various purposes. Moreover, further research is necessary to assess the potency, safety, and pharmacokinetics of CPP-PNAs for clinical prevention and treatment of infections due to H. pylori.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens, Bacterial - genetics
- Bacterial Load - drug effects
- Bacterial Proteins - antagonists & inhibitors, genetics
- Cell Line, Tumor - administration & dosage
- HT29 Cells - administration & dosage
- Helicobacter Infections - drug therapy
- Helicobacter pylori - drug effects, genetics
- Humans - administration & dosage
- Microbial Sensitivity Tests - administration & dosage
- Oligodeoxyribonucleotides, Antisense - genetics, pharmacology
- Peptide Nucleic Acids - genetics, pharmacology
- Real-Time Polymerase Chain Reaction - administration & dosage
- Find related publications in this database (Keywords)
- Helicobacter pylori
- peptide nucleic acid
- antisense
- real-time PCR
- cell culture