Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Werner, ER; Hirsch-Kauffmann, M; Fuchs, D; Hausen, A; Reibnegger, G; Schweiger, M; Wachter, H.
Interferon-gamma-induced degradation of tryptophan by human cells in vitro.
Biol Chem Hoppe Seyler. 1987; 368(10):1407-1412
Doi: 10.1515/bchm3.1987.368.2.1407
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- Co-Autor*innen der Med Uni Graz
- Reibnegger Gilbert
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- Abstract:
- Several human cells were investigated for their ability to degrade tryptophan and to synthesize neopterin upon induction by interferon-gamma (500 units/ml for 48 h). Concentrations of tryptophan, kynurenine, 3-hydroxykynurenine, anthranilic acid, 3-hydroxyanthranilic acid, 7,8-dihydroneopterin and neopterin were assessed in the culture supernatants by HPLC. Fibroblasts, A-22 arachnoidea, HK-2351 scalp, T-2346 meningeom and HeLa cervical carcinoma cells but not HL-60 promyelocytic leukaemia cells were found to degrade tryptophan upon induction by interferon-gamma. Tryptophan is converted to kynurenine by fibroblasts, A-22 arachnoidea and HK-2351 scalp cells and to kynurenine and anthranilic acid by HeLa cervical carcinoma and T-2346 meningeom cells. Kynurenine and anthranilic acid always make up more than 82% of the tryptophan degraded. None of these cells synthesizes 3-hydroxyanthranilic acid, 3-hydroxykynurenine, 7,8-dihydroneopterin or neopterin. Human macrophages form 3-hydroxyanthranilic acid and neopterin, but not 3-hydroxykynurenine, beside kynurenine and anthranilic acid upon activation by interferon-gamma. These data indicate that several human cells can be induced by interferon-gamma to degrade tryptophan. The interferon-gamma induced synthesis of 3-hydroxyanthranilic acid and neopterin, however, appears to be restricted to human macrophages. A hypothesis explaining these findings is presented.
- Find related publications in this database (using NLM MeSH Indexing)
- Anthranilic Acids - analysis
- Biopterin - analogs and derivatives
- Cells, Cultured - analogs and derivatives
- Chromatography, High Pressure Liquid - analogs and derivatives
- Fibroblasts - analogs and derivatives
- Hela Cells - analogs and derivatives
- Humans - analogs and derivatives
- Indicators and Reagents - analogs and derivatives
- Interferon Type II - pharmacology
- Kynurenine - analysis
- Neopterin - analysis
- Tryptophan - analysis