Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Hoch, D; Brandl, W; Strutz, J; Köfeler, HC; van, Poppel, MNM; Bode, L; Hiden, U; Desoye, G; Jantscher-Krenn, E.
Human Milk Oligosaccharides in Cord Blood Are Altered in Gestational Diabetes and Stimulate Feto-Placental Angiogenesis In Vitro.
Nutrients. 2021; 13(12):
Doi: 10.3390/nu13124257
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- Führende Autor*innen der Med Uni Graz
- Brandl Waltraud Theresia
- Hiden Ursula
- Hoch Denise
- Jantscher-Krenn Evelyn
- Co-Autor*innen der Med Uni Graz
- Desoye Gernot
- Köfeler Harald
- Van Poppel Mireille Nicoline Maria
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- Abstract:
- (1) Background: Human milk oligosaccharides (HMOs) are present in maternal serum during pregnancy and their composition is altered in gestational diabetes (GDM). HMOs are also in fetal cord blood and in contact with the feto-placental endothelium, potentially affecting its functions, such as angiogenesis. We hypothesized that cord blood HMOs are changed in GDM and contribute to increased feto-placental angiogenesis, hallmark of GDM. (2) Methods: Using HPLC, we quantified HMOs in cord blood of women with normal glucose tolerance (NGT, n = 25) or GDM (n = 26). We investigated in vitro angiogenesis using primary feto-placental endothelial cells (fpECs) from term placentas after healthy pregnancy (n = 10), in presence or absence of HMOs (100 µg/mL) isolated from human milk, 3'-sialyllactose (3'SL, 30 µg/mL) and lactose (glycan control) and determined network formation (Matrigel assay), proliferation (MTT assays), actin organization (F-actin staining), tube formation (fibrin tube formation assay) and sprouting (spheroid sprouting assay). (3) Results: 3'SL was higher in GDM cord blood. HMOs increased network formation, HMOs and 3'SL increased proliferation and F-actin staining. In fibrin assays, HMOs and 3'SL increased total tube length by 24% and 25% (p < 0.05), in spheroid assays, by 32% (p < 0.05) and 21% (p = 0.056), respectively. Lactose had no effect. (4) Conclusions: Our study suggests a novel role of HMOs in feto-placental angiogenesis and indicates a contribution of HMO composition to altered feto-placental vascularization in GDM.
- Find related publications in this database (Keywords)
- pregnancy
- gestational diabetes mellitus
- human milk oligosaccharides
- angiogenesis
- cord blood
- 3 '-sialyllactose
- placenta
- tube formation
- spheroid sprouting assay
- fibrin tube formation assay