Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Nielson, CM; Liu, CT; Smith, AV; Ackert-Bicknell, CL; Reppe, S; Jakobsdottir, J; Wassel, C; Register, TC; Oei, L; Alonso, N; Oei, EH; Parimi, N; Samelson, EJ; Nalls, MA; Zmuda, J; Lang, T; Bouxsein, M; Latourelle, J; Claussnitzer, M; Siggeirsdottir, K; Srikanth, P; Lorentzen, E; Vandenput, L; Langefeld, C; Raffield, L; Terry, G; Cox, AJ; Allison, MA; Criqui, MH; Bowden, D; Ikram, MA; Mellström, D; Karlsson, MK; Carr, J; Budoff, M; Phillips, C; Cupples, LA; Chou, WC; Myers, RH; Ralston, SH; Gautvik, KM; Cawthon, PM; Cummings, S; Karasik, D; Rivadeneira, F; Gudnason, V; Orwoll, ES; Harris, TB; Ohlsson, C; Kiel, DP; Hsu, YH.
Novel Genetic Variants Associated With Increased Vertebral Volumetric BMD, Reduced Vertebral Fracture Risk, and Increased Expression of SLC1A3 and EPHB2.
J Bone Miner Res. 2016; 31(12): 2085-2097.
Doi: 10.1002/jbmr.2913
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Alonso Lopez Nerea
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Genome-wide association studies (GWASs) have revealed numerous loci for areal bone mineral density (aBMD). We completed the first GWAS meta-analysis (n = 15,275) of lumbar spine volumetric BMD (vBMD) measured by quantitative computed tomography (QCT), allowing for examination of the trabecular bone compartment. SNPs that were significantly associated with vBMD were also examined in two GWAS meta-analyses to determine associations with morphometric vertebral fracture (n = 21,701) and clinical vertebral fracture (n = 5893). Expression quantitative trait locus (eQTL) analyses of iliac crest biopsies were performed in 84 postmenopausal women, and murine osteoblast expression of genes implicated by eQTL or by proximity to vBMD-associated SNPs was examined. We identified significant vBMD associations with five loci, including: 1p36.12, containing WNT4 and ZBTB40; 8q24, containing TNFRSF11B; and 13q14, containing AKAP11 and TNFSF11. Two loci (5p13 and 1p36.12) also contained associations with radiographic and clinical vertebral fracture, respectively. In 5p13, rs2468531 (minor allele frequency [MAF] = 3%) was associated with higher vBMD (β = 0.22, p = 1.9 × 10-8 ) and decreased risk of radiographic vertebral fracture (odds ratio [OR] = 0.75; false discovery rate [FDR] p = 0.01). In 1p36.12, rs12742784 (MAF = 21%) was associated with higher vBMD (β = 0.09, p = 1.2 × 10-10 ) and decreased risk of clinical vertebral fracture (OR = 0.82; FDR p = 7.4 × 10-4 ). Both SNPs are noncoding and were associated with increased mRNA expression levels in human bone biopsies: rs2468531 with SLC1A3 (β = 0.28, FDR p = 0.01, involved in glutamate signaling and osteogenic response to mechanical loading) and rs12742784 with EPHB2 (β = 0.12, FDR p = 1.7 × 10-3 , functions in bone-related ephrin signaling). Both genes are expressed in murine osteoblasts. This is the first study to link SLC1A3 and EPHB2 to clinically relevant vertebral osteoporosis phenotypes. These results may help elucidate vertebral bone biology and novel approaches to reducing vertebral fracture incidence. © 2016 American Society for Bone and Mineral Research.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals - administration & dosage
- Biopsy - administration & dosage
- Bone Density - genetics
- Cancellous Bone - diagnostic imaging, pathology, physiopathology
- Excitatory Amino Acid Transporter 1 - genetics, metabolism
- Gene Expression Regulation - administration & dosage
- Genetic Association Studies - administration & dosage
- Genetic Predisposition to Disease - administration & dosage
- Humans - administration & dosage
- Linkage Disequilibrium - genetics
- Lumbar Vertebrae - diagnostic imaging, pathology, physiopathology
- Mice - administration & dosage
- Molecular Sequence Annotation - administration & dosage
- Organ Size - administration & dosage
- Osteoblasts - metabolism
- Polymorphism, Single Nucleotide - genetics
- Quantitative Trait Loci - genetics
- Receptor, EphB2 - genetics, metabolism
- Risk Factors - administration & dosage
- Spinal Fractures - diagnostic imaging, genetics, pathology, physiopathology
- Spine - diagnostic imaging, pathology
- Find related publications in this database (Keywords)
- BONE QCT
- CT
- ANALYSIS
- QUANTITATION OF BONE
- OSTEOPOROSIS
- DISEASES AND DISORDERS OF
- RELATED TO BONE
- GENERAL POPULATION STUDIES
- EPIDEMIOLOGY
- HUMAN ASSOCIATION STUDIES
- GENETIC RESEARCH
- FRACTURE RISK ASSESSMENT