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Selected Publication:
Lippe, IT; Holzer, P.
Participation of endothelium-derived nitric oxide but not prostacyclin in the gastric mucosal hyperaemia due to acid back-diffusion.
Br J Pharmacol. 1992; 105(3):708-714
Doi: 10.1111/j.1476-5381.1992.tb09043.x
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- Leading authors Med Uni Graz
- Lippe Irmgard Theresia
- Co-authors Med Uni Graz
- Holzer Peter
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- Abstract:
- 1. The possible participation of prostacyclin and nitric oxide (NO) in the gastric mucosal hyperaemic response to acid back-diffusion through a disrupted gastric mucosal barrier was examined. The experiments were carried out on anaesthetized rats in which acid back-diffusion was elicited by gastric perfusion with dilute ethanol in 0.15 M HCl and gastric mucosal blood flow (MBF) was measured by the hydrogen gas clearance technique. 2. Indomethacin (28 mumols kg-1, s.c.), an inhibitor of the formation of cyclo-oxygenase products including prostacyclin, failed to alter mean arterial blood pressure (MAP), basal MBF and the hyperaemic response to acid back-diffusion in urethane-anaesthetized rats. 3. NG-nitro-L-arginine methyl ester (L-NAME; 13 and 43 mumols kg-1, i.v.), an inhibitor of endothelium-derived NO formation, increased MAP in a dose-dependent manner. Whilst basal MBF in urethane-anaesthetized rats was not changed, the increase in MBF caused by gastric perfusion with dilute ethanol in acid was dose-dependently depressed by L-NAME. The loss of H+ ions from the gastric lumen, an indirect measure of acid back-diffusion, was significantly enhanced by 43 mumols kg-1 L-NAME. In contrast, D-NAME (13 and 43 mumols kg-1) was without effect on MAP, basal and stimulated MBF, and acid back-diffusion. 4. Unlike in urethane-anaesthetized rats, L-NAME led to a significant reduction of basal MBF in phenobarbitone-anaesthetized rats. MAP in the phenobarbitone-anaesthetized rats was significantly higher than in urethane-anaesthetized rats, and the hypertensive effect of L-NAME under phenobarbitone anaesthesia was significantly smaller than under urethane anaesthesia.5. The rise in MBF brought about by acid back-diffusion was blocked by L-NAME administered to phenobarbitone-anaesthetized rats. Infusion of L-arginine (120 pmol kg -1 min- ', i.v.) led to a partial, but significant, reversal of the effects of L-NAME on MAP and the hyperaemia due to acid back-diffusion.6. These findings indicate that endothelium-derived NO plays an important mediator role in the gastric mucosal vasodilatation caused by back-diffusion whilst vasodilator prostanoids such as prostacyclin are not involved.
- Find related publications in this database (using NLM MeSH Indexing)
- Anesthesia -
- Animals -
- Arginine - analogs & derivatives
- Blood Pressure - drug effects
- Diffusion -
- Endothelium, Vascular - physiology
- Epoprostenol - physiology
- Female -
- Gastric Acid - physiology
- Gastric Mucosa - blood supply
- Heart Rate - drug effects
- Hyperemia - physiopathology
- Indomethacin - pharmacology
- Male -
- NG-Nitroarginine Methyl Ester -
- Nitric Oxide - metabolism
- Rats -
- Rats, Inbred Strains -
- Regional Blood Flow - physiology
- Urethane -
- Find related publications in this database (Keywords)
- Gastric Mucosal Blood Flow
- Acid Back-Diffusion
- Vasoactive Mediators
- Endothelium-Derived Relaxing Factor
- Prostanoids
- Indomethacin
- Ng-Nitro-L-Arginine Methyl Ester
- L-Arginine