Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Schicho, R; Liebmann, I; Lippe, IT.
Extracellular signal-regulated kinase-1 and -2 are activated by gastric luminal injury in dorsal root ganglion neurons via N-methyl-D-aspartate receptors.
NEUROSCIENCE. 2005; 134(2): 505-514.
Doi: 10.1016/j.neuroscience.2005.04.021
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Schicho Rudolf
- Co-authors Med Uni Graz
- Liebmann Ingrid
- Lippe Irmgard Theresia
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Mitogen activated protein kinases such as phosphorylated extracellular signal-regulated kinase-1 and -2 (pERK 1/2) have been recently demonstrated to play an important role in somatic nociception and hyperalgesia. In the present study we examined whether pERK 1/2 is involved in the response of sensory neurons to a noxious visceral stimulation, in particular, of the gastric mucosa. After induction of gastric injury by oral administration of 0.5M HCl pERK 1/2 expression was determined by Western blotting of caudal thoracic dorsal root ganglia and by immunohistochemistry in stomach-innervating dorsal root ganglion neurons which were retrogradely labeled with True Blue. The content of pERK 1/2 remained unchanged in dorsal root ganglia until 2 h post-HCl, however, was found elevated 4 (approximately 80%) and 6 h (approximately 100%) after HCl administration. True Blue-labeled pERK 1/2-immunoreactive neurons were likewise increased 6 h post-HCl (204%) and were mainly of small size (20-40 microm) and negative for neurofilament 200 (approximately 76%). The majority of these cells also expressed the nociceptive transient receptor potential vanilloid receptor 1 (approximately 70%). The gastric mucosa was simultaneously examined for lesion formation showing highest percentage of damage 6 h post-HCl. Application of a N-methyl-D-aspartate receptor antagonist (MK-801; 100 microg/kg s.c.) significantly reduced HCl-induced pERK 1/2 expression and mucosal lesions 6 h post-HCl. Activation of the extracellular signal-regulated kinase-1 and -2 signaling cascade indicates that visceral primary afferents may sensitize after gastric noxious stimulation involving N-methyl-D-aspartate receptors. The extracellular signal-regulated kinase-1 and -2 pathway therefore may not only be of importance for somatic but also for visceral nociception.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Enzyme Activation -
- Female -
- Ganglia, Spinal - enzymology
- Gastric Mucosa - injuries
- Hydrochloric Acid - toxicity
- Mitogen-Activated Protein Kinase 1 - genetics
- Mitogen-Activated Protein Kinase 3 - genetics
- Neurons - enzymology
- Rats - enzymology
- Rats, Sprague-Dawley - enzymology
- Receptors, N-Methyl-D-Aspartate - physiology
- Stomach - injuries
- Find related publications in this database (Keywords)
- retrograde tracing
- MAP kinase
- TRPV1
- MK-801
- hypersensitivity