Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Koidl, C; Bozic, M; Mossböck, G; Mühlbauer, G; Berg, J; Stöcher, M; Dehnhardt, J; Marth, E; Kessler, HH.
Rapid diagnosis of adenoviral keratoconjunctivitis by a fully automated molecular assay.
OPHTHALMOLOGY. 2005; 112(9): 1521-1528.
Doi: 10.1016/j.ophtha.2005.04.011
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Kessler Harald
- Koidl Christoph
- Co-Autor*innen der Med Uni Graz
- Berg Jörg
- Bozic Michael
- Marth Egon
- Mossböck Georg
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- OBJECTIVE: To establish and evaluate a new test system for rapid detection and diagnosis of adenoviral keratoconjunctivitis. DESIGN: After establishment of the molecular assay, 52 conjunctival smears were studied. PARTICIPANTS: Samples were derived from patients with a clinical presentation compatible with keratoconjunctivitis. METHODS: A molecular assay for detection of human adenovirus (HAdV) based on automated nucleic acid extraction and real time polymerase chain reaction was established and evaluated. The new assay included a heterologous internal control. MAIN OUTCOME MEASURES: Statement about the presence or absence of adenoviral DNA in the specimen. RESULTS: The amplification efficiency was found to be 100%. The detection limit was calculated to be 116 copies per LightCycler capillary. When clinical specimens were tested, 15 of 52 conjunctival smears were found to be positive for HAdV DNA. The internal control was detected in all samples. CONCLUSIONS: The new molecular assay proved to be suitable for rapid diagnosis of adenoviral keratoconjunctivitis in the routine diagnostic laboratory.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenovirus Infections, Human - diagnosis Adenovirus Infections, Human - virology
- Adenoviruses, Human - genetics Adenoviruses, Human - isolation and purification
- Adolescent -
- Adult -
- Aged -
- Aged, 80 and over -
- Automation -
- Child -
- Child, Preschool -
- Conjunctivitis, Viral - diagnosis Conjunctivitis, Viral - virology
- Corneal Ulcer - diagnosis Corneal Ulcer - virology
- DNA Primers - chemistry
- DNA Probes - chemistry
- DNA, Viral - analysis
- Female -
- Gene Amplification -
- Humans -
- Infant -
- Male -
- Middle Aged -
- Reagent Kits, Diagnostic -
- Reverse Transcriptase Polymerase Chain Reaction -