Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Üçal, M; Haindl, MT; Adzemovic, MZ; Zeitelhofer, M; Schaefer, U; Fazekas, F; Hochmeister, S.
Rat Model of Widespread Cerebral Cortical Demyelination Induced by an Intracerebral Injection of Pro-Inflammatory Cytokines.
J Vis Exp. 2021;
Doi: 10.3791/57879
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Haindl Michaela Tanja
- Hochmeister Sonja
- Ücal Muammer
- Co-authors Med Uni Graz
- Fazekas Franz
- Schäfer Ute
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Multiple sclerosis (MS) is the most common immune-mediated disease of the central nervous system (CNS) and progressively leads to physical disability and death, caused by white matter lesions in the spinal cord and cerebellum, as well as by demyelination in grey matter. Whilst conventional models of experimental allergic encephalomyelitis are suitable for the investigation of the cell-mediated inflammation in the spinal and cerebellar white matter, they fail to address grey matter pathologies. Here, we present the experimental protocol for a novel rat model of cortical demyelination allowing the investigation of the pathological and molecular mechanisms leading to cortical lesions. The demyelination is induced by an immunization with low-dose myelin oligodendrocyte glycoprotein (MOG) in an incomplete Freund's adjuvant followed by a catheter-mediated intracerebral delivery of pro-inflammatory cytokines. The catheter, moreover, enables multiple rounds of demyelination without causing injection-induced trauma, as well as the intracerebral delivery of potential therapeutic drugs undergoing a preclinical investigation. The method is also ethically favorable as animal pain and distress or disability are controlled and relatively minimal. The expected timeframe for the implementation of the entire protocol is around 8 - 10 weeks.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals - administration & dosage
- Cerebral Cortex - metabolism
- Cytokines - metabolism
- Encephalomyelitis, Autoimmune, Experimental - chemically induced
- Multiple Sclerosis - administration & dosage
- Myelin-Oligodendrocyte Glycoprotein - administration & dosage
- Rats - administration & dosage