Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kupper, N; Pritz, E; Siwetz, M; Guettler, J; Huppertz, B.
Placental Villous Explant Culture 2.0: Flow Culture Allows Studies Closer to the In Vivo Situation.
Int J Mol Sci. 2021; 22(14):
Doi: 10.3390/ijms22147464
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- Führende Autor*innen der Med Uni Graz
- Huppertz Berthold
- Kupper Nadja Julia
- Co-Autor*innen der Med Uni Graz
- Güttler Jacqueline
- Pritz Elisabeth
- Siwetz Monika
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- Abstract:
- During pregnancy, freely floating placental villi are adapted to fluid shear stress due to placental perfusion with maternal plasma and blood. In vitro culture of placental villous explants is widely performed under static conditions, hoping the conditions may represent the in utero environment. However, static placental villous explant culture dramatically differs from the in vivo situation. Thus, we established a flow culture system for placental villous explants and compared commonly used static cultured tissue to flow cultured tissue using transmission and scanning electron microscopy, immunohistochemistry, and lactate dehydrogenase (LDH) and human chorionic gonadotropin (hCG) measurements. The data revealed a better structural and biochemical integrity of flow cultured tissue compared to static cultured tissue. Thus, this new flow system can be used to simulate the blood flow from the mother to the placenta and back in the most native-like in vitro system so far and thus can enable novel study designs.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Culture Techniques - administration & dosage
- Chorionic Gonadotropin - metabolism
- Chorionic Villi - growth & development, metabolism
- Female - administration & dosage
- Flow Cytometry - administration & dosage
- Humans - administration & dosage
- L-Lactate Dehydrogenase - metabolism
- Pregnancy - administration & dosage
- Trophoblasts - cytology, metabolism
- Find related publications in this database (Keywords)
- flow culture system
- placenta
- explant culture under flow