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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Werner, F; Wagner, C; Simon, M; Glatz, K; Mertz, KD; Laubli, H; Richtig, E; Griss, J; Wagner, SN.
Loss of Lymphotoxin Alpha-Expressing Memory B Cells Correlates with Metastasis of Human Primary Melanoma
DIAGNOSTICS. 2021; 11(7): 1238 Doi: 10.3390/diagnostics11071238 [OPEN ACCESS]
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Co-Autor*innen der Med Uni Graz: Richtig Erika
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Abstract:: Activated antigen-experienced B cells play an unexpected complex role in anti-tumor immunity in human melanoma patients. However, correlative studies between B cell infiltration and tumor progression are limited by the lack of distinction between functional B cell subtypes. In this study, we examined a series of 59 primary and metastatic human cutaneous melanoma specimens with B cell infiltration. Using seven-color multiplex immunohistochemistry and automated tissue imaging and analysis, we analyzed the spatiotemporal dynamics of three major antigen-experienced B cell subpopulations expressing lymphotoxin alpha (LTA/TNFSF1) or interleukin-10 (IL-10) outside tertiary lymphoid structures. The expression of both LTA and IL-10 was not restricted to a particular B cell subtype. In primary melanomas, these cells were predominantly found at the invasive tumor-stroma front and, in metastatic melanomas, they were also found in the intratumoral stroma. In primary melanomas, decreased densities of LTA(+) memory-like and, to a lesser extent, activated B cells were associated with metastasis. Compared with metastatic primary tumors, B cell infiltrates in melanoma metastases were enriched in both LTA(+) memory-like and LTA(+) activated B cells, but not in any of the IL-10(+) B cell subpopulations. Melanoma disease progression shows distinct dynamics of functional B cell subpopulations, with the regulation of LTA(+) B cell numbers being more significant than IL-10(+) B cell subpopulations.
Find related publications in this database (Keywords): tumor-associated B cells; memory B cells; activated B cells; human melanoma; tumor microenvironment; lymphotoxin alpha; Interleukin-10; multiplex immunohistochemistry; spatiotemporal dynamics