Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Gesslbauer, B; Krenn, E; Zenzmaier, C; Preisegger, KH; Kungl, AJ.
Lessons from the stem cell proteome.
Curr Stem Cell Res Ther. 2006; 1(3): 395-409.
Doi: 10.2174/157488806778226867
PubMed
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- Co-Autor*innen der Med Uni Graz
- Jantscher-Krenn Evelyn
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- Abstract:
- The proteome of a cell is a molecular fingerprint directly relating to the gene expression snapshot profile at a certain point of time or developmental stage. Monitoring the expansion and the differentiation state of stem cells by proteomic means seems therefore a very attractive method for diagnostic as well as for therapeutic purposes. We have investigated the protein expression patterns of umbilical cord blood-derived CD34+/AC133+ cells in order to obtain a most comprehensive view of the stem cell proteome. For this purpose, we have applied 2-D gel electrophoresis and 2-D chromatography for most efficient protein/peptide separation and characterisation. The proteins were identified after tryptic digestion by nano-HPLC coupled directly to an ion trap mass spectrometer. An extensive bioinformatic analysis of the protein obtained revealed a dynamic stem cell proteome. This means that the heterogeneity of protein expression patterns obtained from different stem cell preparations refers to a limited set of stem cell-specific house keeping proteins as well as to a large number of proteins which depend on (marginal) stimuli from the environment. Since those are difficult to standardise, snapshot views of the stem cell proteome reflect not only stem cell-intrinsic metabolism but also the strong influence of the sample history on protein expression patterns.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult - administration & dosage
- Child - administration & dosage
- Child, Preschool - administration & dosage
- Cytoskeletal Proteins - genetics, isolation & purification
- Electrophoresis, Gel, Two-Dimensional - administration & dosage
- Embryo, Mammalian - administration & dosage
- Enzymes - genetics, isolation & purification
- Fetal Blood - cytology, physiology
- Humans - administration & dosage
- Infant, Newborn - administration & dosage
- Mass Spectrometry - administration & dosage
- Membrane Proteins - genetics, isolation & purification
- Nuclear Proteins - genetics, isolation & purification
- Proteins - chemistry, genetics, isolation & purification
- Proteomics - administration & dosage
- Stem Cells - physiology