Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Kienesberger, B; Obermüller, B; Singer, G; Mittl, B; Grabherr, R; Mayrhofer, S; Heinl, S; Stadlbauer, V; Horvath, A; Miekisch, W; Fuchs, P; Klymiuk, I; Till, H; Castellani, C.
(S)-Reutericyclin: Susceptibility Testing and In Vivo Effect on Murine Fecal Microbiome and Volatile Organic Compounds.
Int J Mol Sci. 2021; 22(12):
Doi: 10.3390/ijms22126424
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- Führende Autor*innen der Med Uni Graz
- Kienesberger Bernhard
- Obermüller Beate
- Co-Autor*innen der Med Uni Graz
- Castellani Christoph
- Horvath Angela
- Klymiuk Ingeborg
- Mittl Barbara
- Singer Georg
- Stadlbauer-Köllner Vanessa
- Till Holger
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- Abstract:
- We aimed to assess the in vitro antimicrobial activity and the in vivo effect on the murine fecal microbiome and volatile organic compound (VOC) profile of (S)-reutericyclin. The antimicrobial activity of (S)-reutericyclin was tested against Clostridium difficile, Listeria monocytogenes, Escherichia coli, Enterococcus faecium, Staphylococcus aureus, Staphylococcus (S.) epidermidis, Streptococcus agalactiae, Pseudomonas aeruginosa and Propionibacterium acnes. Reutericyclin or water were gavage fed to male BALBc mice for 7 weeks. Thereafter stool samples underwent 16S based microbiome analysis and VOC analysis by gas chromatography mass spectrometry (GC-MS). (S)-reutericyclin inhibited growth of S. epidermidis only. Oral (S)-reutericyclin treatment caused a trend towards reduced alpha diversity. Beta diversity was significantly influenced by reutericyclin. Linear discriminant analysis Effect Size (LEfSe) analysis showed an increase of Streptococcus and Muribaculum as well as a decrease of butyrate producing Ruminoclostridium, Roseburia and Eubacterium in the reutericyclin group. VOC analysis revealed significant increases of pentane and heptane and decreases of 2,3-butanedione and 2-heptanone in reutericyclin animals. The antimicrobial activity of (S)-reutericyclin differs from reports of (R)-reutericyclin with inhibitory effects on a multitude of Gram-positive bacteria reported in the literature. In vivo (S)-reutericyclin treatment led to a microbiome shift towards dysbiosis and distinct alterations of the fecal VOC profile.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals - administration & dosage
- Discriminant Analysis - administration & dosage
- Feces - microbiology
- Male - administration & dosage
- Mice, Inbred BALB C - administration & dosage
- Microbial Sensitivity Tests - administration & dosage
- Microbiota - drug effects
- Tenuazonic Acid - analogs & derivatives, pharmacology
- Volatile Organic Compounds - analysis
- Find related publications in this database (Keywords)
- reutericyclin
- bacteriocin
- microbiome
- antimicrobial activity
- VOC
- isoform
- antibiotic
- resistance