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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Wolters, F; Grunberg, M; Huber, M; Kessler, HH; Pruller, F; Saleh, L; Febreau, C; Rahamat-Langendoen, J; Thibault, V; Melchers, WJG.
European multicenter evaluation of Xpert (R) Xpress SARS-CoV-2/Flu/RSV test
J MED VIROL. 2021; Doi: 10.1002/jmv.27111 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

Co-Autor*innen der Med Uni Graz: Kessler Harald; Prüller Florian
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Abstract:: Rapid diagnostics for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are paramount for reducing the spread of the current pandemic. During additional seasonal epidemics with influenza A/B and respiratory syncytial virus (RSV), the clinical signs and symptoms cannot be distinguished easily from SARS-CoV-2. Therefore, a new assay combining four targets in the form of the new Xpert Xpress SARS-CoV-2/Flu/RSV assay was evaluated. The assay was compared to the Xpert Xpress SARS-CoV-2, Xpert Xpress Flu/RSV, Seegene Flu/RSV, influenza A/B r-gene (R) and RSV/hMPV r-gene (R). A total of 295 nasopharyngeal and throat swabs were tested at four institutes throughout Europe including 72 samples positive for SARS-CoV-2, 65 for influenza A, 47 for influenza B, and 77 for RSV. The sensitivity of the new assay was above 95% for all targets, with the highest for SARS-CoV-2 (97.2%). The overall correlation of SARS-CoV-2 Ct values between Xpert Xpress SARS-CoV-2 assay and Xpert Xpress SARS-CoV-2/Flu/RSV assay was high. The agreement between Ct values above 30 showed the multiplex giving higher Ct values for SARS-CoV-2 on average than the singleplex assay. In conclusion, the new assay is a rapid and reliable alternative with less hands-on time for the detection of not one, but four upper respiratory tract pathogens that may circulate at the same time.
Find related publications in this database (Keywords): COVID-19; influenza; multiplex PCR; pandemic; respiratory syncytial virus; SARS-CoV-2