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SHR Neuro Cancer Cardio Lipid Metab Microb

Meindl, C; Öhlinger, K; Zrim, V; Steinkogler, T; Fröhlich, E.
Screening for Effects of Inhaled Nanoparticles in Cell Culture Models for Prolonged Exposure.
Nanomaterials (Basel). 2021; 11(3): Doi: 10.3390/nano11030606 [OPEN ACCESS]
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Leading authors Med Uni Graz: Fröhlich Eleonore; Meindl Claudia
Co-authors Med Uni Graz: Öhlinger Kristin Anna; Zrim Verena
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Abstract:: Respiratory exposure of humans to environmental and therapeutic nanoparticles repeatedly occurs at relatively low concentrations. To identify adverse effects of particle accumulation under realistic conditions, monocultures of Calu-3 and A549 cells and co-cultures of A549 and THP-1 macrophages in the air-liquid interphase culture were exposed repeatedly to 2 µg/cm² 20 nm and 200 nm polystyrene particles with different functionalization. Particle accumulation, transepithelial electrical resistance, dextran (3-70 kDa) uptake and proinflammatory cytokine secretion were determined over 28 days. Calu-3 cells showed constant particle uptake without any change in barrier function and cytokine release. A549 cells preferentially ingested amino- and not-functionalized particles combined with decreased endocytosis. Cytokine release was transiently increased upon exposure to all particles. Carboxyl-functionalized demonstrated higher uptake and higher cytokine release than the other particles in the A549/THP-1 co-cultures. The evaluated respiratory cells and co-cultures ingested different amounts and types of particles and caused small (partly transient) effects. The data suggest that the healthy cells can adapt to low doses of non-cytotoxic particles.
Find related publications in this database (Keywords): nanotoxicity; respiratory exposure; in vitro models; polystyrene particles; macrophages; cytotoxicity; repeated exposure; air–; liquid interface; proinflammatory effects