Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Cardenas, G; Menger, MFSJ; Ramos-Berdullas, N; Sánchez-Murcia, PA.
Deciphering the Chemical Basis of Fluorescence of a Selenium-Labeled Uracil Probe when Bound at the Bacterial Ribosomal A-Site.
Chemistry. 2021; 27(15):4927-4931
Doi: 10.1002/chem.202004818
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Sánchez Murcia Pedro Alejandro
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- Abstract:
- We unveil in this work the main factors that govern the turn-on/off fluorescence of a Se-modified uracil probe at the ribosomal RNA A-site. Whereas the constraint into an "in-plane" conformation of the two rings of the fluorophore is the main driver for the observed turn-on fluorescence emission in the presence of the antibiotic paromomycin, the electrostatics of the environment plays a minor role during the emission process. Our computational strategy clearly indicates that, in the absence of paromomycin, the probe prefers conformations that show a dark S1 electronic state with participation of nπ* electronic transition contributions between the selenium atom and the π-system of the uracil moiety. © 2020 Wiley-VCH GmbH.
- Find related publications in this database (using NLM MeSH Indexing)
- Fluorescence -
- Molecular Conformation -
- Ribosomes -
- Selenium -
- Uracil -
- Find related publications in this database (Keywords)
- antibiotics
- excited states
- fluorescence probes
- QM
- MM
- RNA