Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Coué, G; Freese, C; Unger, RE; Kirkpatrick, CJ; Pickl, KE; Sinner, FM; Engbersen, JF.
Design and physicochemical characterization of poly(amidoamine) nanoparticles and the toxicological evaluation in human endothelial cells: applications to peptide delivery to the brain.
J Biomater Sci Polym Ed. 2013; 24(8): 957-971.
Doi: 10.1080/09205063.2012.727378
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Sinner Frank Michael
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- Abstract:
- In this study, we investigated nanoparticles formulated by self-assembly of a biodegradable poly(amidoamine) (PAA) and a fluorescently labeled peptide, in their capacity to internalize in endothelial cells and deliver the peptide, with possible applications for brain drug delivery. The nanoparticles were characterized in terms of size, surface charge, and loading efficiency, and were applied on human cerebral microvascular endothelial cells (hCMEC/D3) and human umbilical vein endothelial cells (Huvec) cells. Cell-internalization and cytotoxicity experiments showed that the PAA-based nanocomplexes were essentially nontoxic, and the peptide was successfully internalized into cells. The results indicate that these PAAs have an excellent property as nontoxic carriers for intracellular protein and peptide delivery, and provide opportunities for novel applications in the delivery of peptides to endothelial cells of the brain.
- Find related publications in this database (using NLM MeSH Indexing)
- Brain - metabolism
- Cells, Cultured -
- Drug Carriers - chemistry
- Endothelial Cells - drug effects
- Endothelial Cells - metabolism
- Human Umbilical Vein Endothelial Cells -
- Humans -
- Microvessels - cytology
- Nanoparticles - chemistry
- Nanoparticles - toxicity
- Oligopeptides - administration & dosage
- Oligopeptides - chemistry
- Polyamines - chemistry
- Polyamines - toxicity
- Find related publications in this database (Keywords)
- poly(amidoamine)s
- nanoparticle
- bloodbrain barrier
- peptide delivery