Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Lembke, M; Pennetzdorfer, N; Tutz, S; Koller, M; Vorkapic, D; Zhu, J; Schild, S; Reidl, J.
Proteolysis of ToxR is controlled by cysteine-thiol redox state and bile salts in Vibrio cholerae.
Mol Microbiol. 2018; 110(5): 796-810.
Doi: 10.1111/mmi.14125
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- Co-Autor*innen der Med Uni Graz
- Koller Michael
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- Abstract:
- In Vibrio cholerae, virulence gene expression is regulated by a transmembrane-localized transcription factor complex designated as ToxRS. ToxR harbours two cysteines in the periplasmic domain that can form inter- and intramolecular disulfide bonds. In this study, we investigated the σE -dependent inner membrane proteolysis of ToxR, which occurs via the periplasmic-localized proteases DegS and DegP. Both proteases respond to the redox state of the two cysteine thiol groups of ToxR. Interestingly, in the presence of sodium deoxycholate, ToxR proteolysis is blocked independently of ToxS, whereas ToxR activation by bile salts requires ToxS function. From these data, we identified at least two levels of control for ToxR activation by sodiumdeoxycholate. First, bile inhibits ToxR degradation under starvation and alkaline pH or under conditions in which DegPS responds to the reduced disulfide bonds of ToxR. The second level links bile to ToxRS complex formation and further activation of its transcription factor activity. Overall, our data suggest a comprehensive bile sensory function for the ToxRS complex during host colonization. © 2018 The Authors. Molecular Microbiology Published by John Wiley & Sons Ltd.
- Find related publications in this database (using NLM MeSH Indexing)
- Bacterial Proteins - metabolism
- Bile Acids and Salts - metabolism
- Cysteine - metabolism
- DNA-Binding Proteins - metabolism
- Gene Expression Regulation, Bacterial -
- Heat-Shock Proteins - metabolism
- Membrane Proteins - metabolism
- Oxidation-Reduction -
- Periplasm - metabolism
- Periplasmic Proteins - metabolism
- Protein Domains -
- Proteolysis -
- Serine Endopeptidases - metabolism
- Sulfhydryl Compounds - metabolism
- Transcription Factors - metabolism
- Vibrio cholerae - metabolism