Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Üçal, M; Maurer, C; Etschmaier, V; Hamberger, D; Grünbacher, G; Tögl, L; Roosen, MJ; Molcanyi, M; Vorholt, D; Hatay, FF; Hescheler, J; Pallasch, C; Schaefer, U; Patz, S.
Inflammatory Pre-conditioning of Adipose Derived Stem Cells (ADSCs) with Cerebrospinal Fluid (CSF) from Traumatic Brain Injury Patients Alters the Immunomodulatory Potential of ADSC Secretomes.
J Neurotrauma. 2021;
Doi: 10.1089/neu.2020.7017
Web of Science
PubMed
FullText
FullText_MUG
- Leading authors Med Uni Graz
- Patz Silke
- Ücal Muammer
- Co-authors Med Uni Graz
- Etschmaier Vanessa
- Grünbacher Gerda
- Hamberger Daniel
- Maurer Christa
- Molcanyi Marek
- Roosen Marvin Johannes
- Schäfer Ute
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- Abstract:
- Immunomodulation by adipose tissue-derived stem cells (ADSCs) is of special interest for the alleviation of damaging inflammatory responses in central nervous system injuries. The present study explored the effects of cerebrospinal fluid (CSF) from traumatic brain injury patients (TBI) on this immunomodulatory potential of ADSCs. CSF conditioning of ADSCs increased mRNA levels of both pro- and anti-inflammatory genes compared to controls. Exposure of phorbol-12-myristate-13-acetate (PMA)-differentiated THP1 macrophages to the secretome of CSF conditioned ADSCs downregulated both pro-inflammatory (COX-2, TNFα) and anti-inflammatory (SOCS3, IL1RA, TGFβ) genes in these cells. IL-10 expression was elevated in both naïve and conditioned secretomes. ADSC secretome treatment, further, induced macrophage maturation of THP1 cells and increased the percentage of CD11b+, CD14+, CD86+ and, to a lesser extent, CD206+ cells. This, moreover, enhanced the phagocytic activity of CD14+ and CD86+ cells, though independently of pre-conditioning. Secretome exposure, finally, also induced a reduction in the percentage of CD192+ adherent cells in cultures of peripheral blood mononuclear cells (PBMCs) from both healthy subjects and TBI patients. This limited efficacy (of both naïve and pre-conditioned secretomes) suggests that the effects of lymphocyte-monocyte paracrine signalling on the fate of cultured PBMC are strongest upon adherent cell populations.
- Find related publications in this database (Keywords)
- adipose-derived stem cells
- conditioning
- inflammation
- secretome
- traumatic brain injury
- immunomodulation