Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Stentzel, S; Teufelberger, A; Nordengrün, M; Kolata, J; Schmidt, F; van Crombruggen, K; Michalik, S; Kumpfmüller, J; Tischer, S; Schweder, T; Hecker, M; Engelmann, S; Völker, U; Krysko, O; Bachert, C; Bröker, BM.
Staphylococcal serine protease-like proteins are pacemakers of allergic airway reactions to Staphylococcus aureus.
J Allergy Clin Immunol. 2017; 139(2): 492-500.
Doi: 10.1016/j.jaci.2016.03.045
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Teufelberger Andrea Renate
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- Abstract:
- A substantial subgroup of asthmatic patients have "nonallergic" or idiopathic asthma, which often takes a severe course and is difficult to treat. The cause might be allergic reactions to the gram-positive pathogen Staphylococcus aureus, a frequent colonizer of the upper airways. However, the driving allergens of S aureus have remained elusive. We sought to search for potentially allergenic S aureus proteins and characterize the immune response directed against them. S aureus extracellular proteins targeted by human serum IgG4 were identified by means of immunoblotting to screen for potential bacterial allergens. Candidate antigens were expressed as recombinant proteins and used to analyze the established cellular and humoral immune responses in healthy adults and asthmatic patients. The ability to induce a type 2 immune response in vivo was tested in a mouse asthma model. We identified staphylococcal serine protease-like proteins (Spls) as dominant IgG4-binding S aureus proteins. SplA through SplF are extracellular proteases of unknown function expressed by S aureus in vivo. Spls elicited IgE antibody responses in most asthmatic patients. In healthy S aureus carriers and noncarriers, peripheral blood T cells elaborated TH2 cytokines after stimulation with Spls, as is typical for allergens. In contrast, TH1/TH17 cytokines, which dominated the response to S aureus α-hemolysin, were of low concentration or absent. In mice inhalation of SplD without adjuvant induced lung inflammation characterized by TH2 cytokines and eosinophil infiltration. We identify Spls as triggering allergens released by S aureus, opening prospects for diagnosis and causal therapy of asthma. Copyright © 2016 The Authors. Published by Elsevier Inc. All rights reserved.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Aged -
- Allergens - immunology
- Allergens - metabolism
- Animals -
- Asthma - immunology
- Bacterial Proteins - immunology
- Bacterial Proteins - metabolism
- Cells, Cultured -
- Female -
- Humans -
- Hypersensitivity - immunology
- Immunoglobulin E - metabolism
- Immunoglobulin G - metabolism
- Lymphocyte Activation -
- Male -
- Mice -
- Mice, Inbred C57BL -
- Middle Aged -
- Protein Binding -
- Serine Proteases - immunology
- Staphylococcal Infections - immunology
- Staphylococcus aureus - immunology
- Th2 Cells - immunology
- Young Adult -
- Find related publications in this database (Keywords)
- Asthma
- IgE
- Staphylococcus aureus
- serine proteaselike proteins
- type 2 inflammation