Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Eylert, G; Dolp, R; Parousis, A; Cheng, R; Auger, C; Holter, M; Lang-Olip, I; Reiner, V; Kamolz, LP; Jeschke, MG.
Skin regeneration is accelerated by a lower dose of multipotent mesenchymal stromal/stem cells-a paradigm change.
Stem Cell Res Ther. 2021; 12(1): 82-82.
Doi: 10.1186/s13287-020-02131-6
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- Führende Autor*innen der Med Uni Graz
- Eylert Gertraud
- Co-Autor*innen der Med Uni Graz
- Holter Magdalena
- Kamolz Lars-Peter
- Lang-Olip Ingrid
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- Abstract:
- Multipotent mesenchymal stromal/stem cell (MSC) therapy is under investigation in promising (pre-)clinical trials for wound healing, which is crucial for survival; however, the optimal cell dosage remains unknown. The aim was to investigate the efficacy of different low-to-high MSC dosages incorporated in a biodegradable collagen-based dermal regeneration template (DRT) Integra®. We conducted a porcine study (N = 8 Yorkshire pigs) and seeded between 200 and 2,000,000 cells/cm2 of umbilical cord mesenchymal stromal/stem cells on the DRT and grafted it onto full-thickness burn excised wounds. On day 28, comparisons were made between the different low-to-high cell dose groups, the acellular control, a burn wound, and healthy skin. We found that the low dose range between 200 and 40,000 cells/cm2 regenerates the full-thickness burn excised wounds most efficaciously, followed by the middle dose range of 200,000-400,000 cells/cm2 and a high dose of 2,000,000 cells/cm2. The low dose of 40,000 cells/cm2 accelerated reepithelialization, reduced scarring, regenerated epidermal thickness superiorly, enhanced neovascularization, reduced fibrosis, and reduced type 1 and type 2 macrophages compared to other cell dosages and the acellular control. This regenerative cell therapy study using MSCs shows efficacy toward a low dose, which changes the paradigm that more cells lead to better wound healing outcome.
- Find related publications in this database (Keywords)
- Skin regeneration
- Multipotent mesenchymal stromal
- stem cells
- Wound healing
- Cell therapy
- Tissue engineering
- Integra
- Umbilical cord mesenchymal stromal
- stem cells
- Experimental surgery
- Skin substitutes