Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Cosi, E; Abidalla, MT; Roversi, PF.
The effect of Tween 80 on eggshell permeabilization in Galleria mellonella (L.) (Lepidoptera, Pyralidae).
Cryo Letters. 2010; 31(4):291-300
Web of Science
PubMed
- Co-Autor*innen der Med Uni Graz
- Abidalla Muhamad
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- Abstract:
- The development of a species-specific protocol for dechorionation and permeabilization of insect eggs is a necessary prerequisite to cryopreserve the embryos. Here we tested different procedures based on heptane or the surfactant Tween 80 as an alternative to alkane, evaluating their efficacy and toxicity on the early (24 h post-oviposition) and late (75 h post-oviposition) stage embryos. Heptane efficiently permeabilized the eggs of G. mellonella but the hatching rate ranged from 0.1 to 4.2 percent in the early stage and from 4.3 to 11.2 percent in the late stage. The embryos treated with 1.25 percent NaOCl + 0.08 percent Tween 80 for 2 min showed the same shrinkage and reswelling percentages as eggs exposed to heptane for 10 sec, with a significantly higher hatching percentage in the early (68.2 +/- 1.5 percent) and late stages (22.4 +/- 3.7 percent). Thus, 0.08 percent Tween 80 allows sufficient permeabilization of G. mellonella embryos without the high toxicity of alkane.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cell Membrane Permeability - drug effects
- Cryopreservation - methods
- Cryoprotective Agents - pharmacokinetics
- Heptanes - pharmacology
- Lepidoptera - embryology
- Ovum - drug effects
- Ovum - metabolism
- Polysorbates - pharmacology
- Sodium Hypochlorite - pharmacology
- Surface-Active Agents - pharmacology
- Thiosulfates - pharmacology
- Vitelline Membrane - drug effects
- Vitelline Membrane - metabolism
- Find related publications in this database (Keywords)
- Galleria mellonella
- eggshell
- dechorionation
- permeabilization
- Tween 80
- cryopreservation