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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Abidalla, M; Battaglia, D.
Novel staining procedures for whole mount preparations of greater wax moth (Galleria mellonella) embryos: toluidine blue-rhodamine B, plus calcofluor white.
Biotech Histochem. 2019; 94(2):108-114
Doi: 10.1080/10520295.2018.1519726
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- Leading authors Med Uni Graz
- Abidalla Muhamad
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- Abstract:
- Although hematoxylin and eosin (H & E) staining of sectioned embryonic insect material is widely used, it is time-consuming and may not provide sufficient information. We evaluated new staining procedures for embryonic whole mounts of the greater wax moth, Galleria mellonella. We compared a combination of toluidine blue and rhodamine B (TB-RB) to H & E; we also investigated calcofluor white (CFW) staining. TB-RB staining produced staining similar to H & E. TB-RB staining was less time-consuming and improved visualization of the blastoderm and its differentiation into the germ disk and serosa membrane. CFW enhanced details of mitosis in nuclei post-fertilization and stained the primary serosal membrane. Staining of whole mounts with TB-RB and CFW enabled embryonic staging that was more rapid, convenient and effective than the routine approach using H & E and fluorescent probes.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Benzenesulfonates -
- Hematoxylin -
- Moths -
- Rhodamines -
- Staining and Labeling - methods
- Tolonium Chloride -
- Find related publications in this database (Keywords)
- Calcofluor white
- embryogenesis
- Galleria mellonella
- hematoxylin and eosin
- rhodamine B
- toluidine blue