Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Maity, D; Gigante, A; Sánchez-Murcia, PA; Sijbesma, E; Li, M; Bier, D; Mosel, S; Knauer, S; Ottmann, C; Schmuck, C.
Arginine mimetic appended peptide-based probes for fluorescence turn-on detection of 14-3-3 proteins.
Org Biomol Chem. 2019; 17(17):4359-4363
Doi: 10.1039/c9ob00620f
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Sánchez Murcia Pedro Alejandro
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- Abstract:
- 14-3-3 proteins are adaptor elements in intracellular signaling pathways. Recently, this protein family has been identified as a relevant therapeutic target involved in many human diseases. Therefore, identification of 14-3-3 proteins in biological systems is very important. Two cationic peptide-based probes are reported for the fluorescence detection of 14-3-3 proteins at physiological pH. The design of these probes consists of two symmetric peptidic arms equipped with a guanidiniocarbonyl pyrrole moiety (an arginine mimetic aka GCP), and an environment-sensitive amino-naphthalimide fluorophore as a third arm. These peptide sequences also contain lysine and phenylalanine/tryptophan amino acids for additional charge-charge and hydrophobic interactions. Both probes show high affinity and sensitivity for the 14-3-3 family, as well as good selectivity against other relevant biological proteins and ions.
- Find related publications in this database (using NLM MeSH Indexing)
- 14-3-3 Proteins - analysis
- Arginine - chemistry
- Fluorescent Dyes - chemistry
- Humans -
- Models, Molecular -
- Molecular Structure -
- Peptides - chemistry