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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Del Arco, J; Sánchez-Murcia, PA; Mancheño, JM; Gago, F; Fernández-Lucas, J.
Characterization of an atypical, thermostable, organic solvent- and acid-tolerant 2'-deoxyribosyltransferase from Chroococcidiopsis thermalis.
Appl Microbiol Biotechnol. 2018; 102(16):6947-6957
Doi: 10.1007/s00253-018-9134-y
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- Co-authors Med Uni Graz
- Sánchez Murcia Pedro Alejandro
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- Abstract:
- In our search for thermophilic and acid-tolerant nucleoside 2'-deoxyribosyltransferases (NDTs), we found a good candidate in an enzyme encoded by Chroococcidiopsis thermalis PCC 7203 (CtNDT). Biophysical and biochemical characterization revealed CtNDT as a homotetramer endowed with good activity and stability at both high temperatures (50-100 °C) and a wide range of pH values (from 3 to 7). CtNDT recognizes purine bases and their corresponding 2'-deoxynucleosides but is also proficient using cytosine and 2'-deoxycytidine as substrates. These unusual features preclude the strict classification of CtNDT as either a type I or a type II NDT and further suggest that this simple subdivision may need to be updated in the future. Our findings also hint at a possible link between oligomeric state and NDT's substrate specificity. Interestingly from a practical perspective, CtNDT displays high activity (80-100%) in the presence of several water-miscible co-solvents in a proportion of up to 20% and was successfully employed in the enzymatic production of several therapeutic nucleosides such as didanosine, vidarabine, and cytarabine.
- Find related publications in this database (using NLM MeSH Indexing)
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- Enzymes, Immobilized - metabolism
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- Pentosyltransferases - biosynthesis
- Pentosyltransferases - chemistry
- Pentosyltransferases - genetics
- Pentosyltransferases - isolation & purification
- Solvents - chemistry
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- Find related publications in this database (Keywords)
- Enzymatic synthesis
- Nucleoside analogues
- Nucleoside 2 '-deoxyribosyltransferase
- Extremophiles
- Homology modeling