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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Benavente, R; Esteban-Torres, M; Kohring, GW; Cortés-Cabrera, Á; Sánchez-Murcia, PA; Gago, F; Acebrón, I; de las Rivas, B; Muñoz, R; Mancheño, JM.
Enantioselective oxidation of galactitol 1-phosphate by galactitol-1-phosphate 5-dehydrogenase from Escherichia coli.
Acta Crystallogr D Biol Crystallogr. 2015; 71(Pt 7):1540-1554
Doi: 10.1107/S1399004715009281
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- Co-authors Med Uni Graz
- Sánchez Murcia Pedro Alejandro
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- Abstract:
- Galactitol-1-phosphate 5-dehydrogenase (GPDH) is a polyol dehydrogenase that belongs to the medium-chain dehydrogenase/reductase (MDR) superfamily. It catalyses the Zn(2+)- and NAD(+)-dependent stereoselective dehydrogenation of L-galactitol 1-phosphate to D-tagatose 6-phosphate. Here, three crystal structures of GPDH from Escherichia coli are reported: that of the open state of GPDH with Zn(2+) in the catalytic site and those of the closed state in complex with the polyols Tris and glycerol, respectively. The closed state of GPDH reveals no bound cofactor, which is at variance with the conformational transition of the prototypical mammalian liver alcohol dehydrogenase. The main intersubunit-contacting interface within the GPDH homodimer presents a large internal cavity that probably facilitates the relative movement between the subunits. The substrate analogue glycerol bound within the active site partially mimics the catalytically relevant backbone of galactitol 1-phosphate. The glycerol binding mode reveals, for the first time in the polyol dehydrogenases, a pentacoordinated zinc ion in complex with a polyol and also a strong hydrogen bond between the primary hydroxyl group and the conserved Glu144, an interaction originally proposed more than thirty years ago that supports a catalytic role for this acidic residue.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
- Catalytic Domain -
- Cations, Divalent - metabolism
- Crystallography, X-Ray -
- Escherichia coli - chemistry
- Escherichia coli - metabolism
- Escherichia coli Proteins - chemistry
- Escherichia coli Proteins - metabolism
- Glycerol - metabolism
- Models, Molecular -
- Molecular Sequence Data -
- NAD - metabolism
- NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases - chemistry
- NAD (+) and NADP (+) Dependent Alcohol Oxidoreductases - metabolism
- Oxidation-Reduction -
- Protein Conformation -
- Sequence Alignment -
- Stereoisomerism -
- Tromethamine - metabolism
- Zinc - metabolism
- Find related publications in this database (Keywords)
- crystal structure
- galactitol-1-phosphate 5-dehydrogenase
- metal binding
- enantioselectivity
- galactitol phosphate
- dehydrogenase
- metal coordination
- catalytic mechanism