Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Farinazzo, A; Angiari, S; Turano, E; Bistaffa, E; Dusi, S; Ruggieri, S; Bonafede, R; Mariotti, R; Constantin, G; Bonetti, B.
Nanovesicles from adipose-derived mesenchymal stem cells inhibit T lymphocyte trafficking and ameliorate chronic experimental autoimmune encephalomyelitis.
Sci Rep. 2018; 8(1): 7473-7473.
Doi: 10.1038/s41598-018-25676-2
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- Führende Autor*innen der Med Uni Graz
- Angiari Stefano
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- Abstract:
- Cell based-therapies represent promising strategies for the treatment of neurological diseases. We have previously shown that adipose stem cells (ASC) ameliorate chronic experimental autoimmune encephalomyelitis (EAE). Recent evidence indicates that most ASC paracrine effects are mediated by extracellular vesicles, i.e. micro- and nanovesicles (MVs and NVs). We show that preventive intravenous administration of NVs isolated from ASC (ASC-NVs) before disease onset significantly reduces the severity of EAE and decreases spinal cord inflammation and demyelination, whereas therapeutic treatment with ASC-NVs does not ameliorate established EAE. This treatment marginally inhibits antigen-specific T cell activation, while reducing microglial activation and demyelination in the spinal cord. Importantly, ASC-NVs inhibited integrin-dependent adhesion of encephalitogenic T cells in vitro, with no effect on adhesion molecule expression. In addition, intravital microscopy showed that encephalitogenic T cells treated with ASC NVs display a significantly reduced rolling and firm adhesion in inflamed spinal cord vessels compared to untreated cells. Our results show that ASC-NVs ameliorate EAE pathogenesis mainly by inhibiting T cell extravasation in the inflamed CNS, suggesting that NVs may represent a novel therapeutic approach in neuro-inflammatory diseases, enabling the safe administration of ASC effector factors.
- Find related publications in this database (using NLM MeSH Indexing)
- Adipose Tissue - cytology
- Animals -
- Cell Movement - immunology
- Cells, Cultured -
- Chronic Disease -
- Encephalomyelitis, Autoimmune, Experimental - immunology
- Encephalomyelitis, Autoimmune, Experimental - pathology
- Encephalomyelitis, Autoimmune, Experimental - therapy
- Extracellular Vesicles - physiology
- Extracellular Vesicles - transplantation
- Mesenchymal Stem Cell Transplantation - methods
- Mesenchymal Stem Cells - cytology
- Mice -
- Mice, Inbred C57BL -
- Mice, Transgenic -
- T-Lymphocytes - pathology
- T-Lymphocytes - physiology