Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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SHR Neuro Krebs Kardio Lipid Stoffw Microb

Rauter, T; Burgstaller, S; Gottschalk, B; Ramadani-Muja, J; Bischof, H; Hay, JC; Graier, WF; Malli, R.
ER-to-Golgi Transport in HeLa Cells Displays High Resilience to Ca²⁺ and Energy Stresses.
Cells. 2020; 9(10): Doi: 10.3390/cells9102311 [OPEN ACCESS]
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Führende Autor*innen der Med Uni Graz: Malli Roland; Rauter Thomas
Co-Autor*innen der Med Uni Graz: Bischof Helmut; Burgstaller Sandra; Gottschalk Benjamin; Graier Wolfgang; Ramadani-Muja Jeta
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Abstract:: One third of all human proteins are either transmembrane or soluble secretory proteins that first target the endoplasmic reticulum (ER). These proteins subsequently leave the ER and enter the Golgi apparatus via ER-Golgi intermediate vesicular structures. Live-cell imaging of cargos fused to fluorescent proteins (FPs) enables the high-resolution visualization and characterization of secretory transport processes. Here, we performed fluorescence time-lapse imaging to assess the Ca²⁺ and energy dependency of ER-to-Golgi transport in living HeLa cells, a cancer cell model which has been well investigated. Our data revealed that ER-to-Golgi transport remained highly efficient in the absence of ATP-generating substrates, despite clear reductions in cytosolic and mitochondrial ATP levels under these energy stress conditions. However, cell treatment with 2-deoxy-D-glucose (2-DG), which severely diminished subcellular ATP levels, abolished ER-to-Golgi transport. Interestingly, while 2-DG elevated cytosolic Ca²⁺ levels and reduced long-distance movements of glycosylphosphatidylinositol (GPI)-positive vesicles, robust short-term ER Ca²⁺ mobilizations, which strongly affected the motility of these vesicles, did not considerably impair ER-to-Golgi transport. In summary, we highlight that ER-to-Golgi transport in HeLa cells remains functional despite high energy and Ca²⁺ stress levels.
Find related publications in this database (Keywords): ER-to-Golgi transport; coat protein complex II (COPII) vesicles; vesicle trafficking; cancer cell metabolism; cellular calcium homeostasis; fluorescent protein technology; live-cell imaging; protein transport and sorting; secretory pathway; subcellular ATP imaging