Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Djalinac, N; Ljubojevic-Holzer, S; Matzer, I; Kolesnik, E; Jandl, K; Lohberger, B; Rainer, P; Heinemann, A; Sedej, S; von, Lewinski, D; Bisping, E.
The role of stretch, tachycardia and sodium-calcium exchanger in induction of early cardiac remodelling.
J Cell Mol Med. 2020; 24(15):8732-8743
Doi: 10.1111/jcmm.15504
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- Führende Autor*innen der Med Uni Graz
- Dalinac Natasa
- von Lewinski Dirk
- Co-Autor*innen der Med Uni Graz
- Bisping Egbert Hubertus
- Heinemann Akos
- Holzer Senka
- Jandl Katharina
- Kolesnik Ewald
- Lohberger Birgit
- Matzer Ingrid
- Rainer Peter
- Sedej Simon
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- Abstract:
- Stretch and tachycardia are common triggers for cardiac remodelling in various conditions, but a comparative characterization of their role in the excitation-transcription coupling (ETC) and early regulation of gene expression and structural changes is lacking. Here, we show that stretch and tachycardia directly induced hypertrophy of neonatal rat cardiac myocytes and also of non-myocytes. Both triggers induced similar patterns of hypertrophy but had largely distinct gene expression profiles. ACTA1 served as good hypertrophy marker upon stretch, while RCAN1 was found increased in response to tachycardia in a rate-dependent fashion. Mechanistically, several calcium-handling proteins, including the sodium-calcium exchanger (NCX), contributed to ETC. Phosphorylation of the calcium/calmodulin-dependent protein kinase II (CaMKII) was elevated and occurred downstream of NCX activation upon tachycardia, but not stretch. Microarray profiling revealed that stretch and tachycardia regulated around 33% and 20% genes in a NCX-dependent manner, respectively. In conclusion, our data show that hypertrophy induction by stretch and tachycardia is associated with different gene expression profiles with a significant contribution of the NCX.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals - administration & dosage
- Calcium - metabolism
- Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism
- Cells, Cultured - administration & dosage
- Disease Models, Animal - administration & dosage
- Disease Susceptibility - administration & dosage
- Excitation Contraction Coupling - administration & dosage
- Gene Expression Regulation - administration & dosage
- Myocytes, Cardiac - metabolism, pathology
- Phosphorylation - administration & dosage
- Rats - administration & dosage
- Sodium-Calcium Exchanger - genetics, metabolism
- Tachycardia - complications, diagnosis, etiology
- Transcription, Genetic - administration & dosage
- Ventricular Remodeling - genetics
- Find related publications in this database (Keywords)
- Calcium
- Calmodulin-dependent protein kinase II (CaMKII)
- cardiac remodelling
- ET coupling (ETC)
- hypertrophy
- mechanical stretch
- neonatal rat ventricular cardiomyocytes (NRVCM)
- RNA expression
- sodium-calcium exchanger (NCX)
- tachycardia