Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Grossmann, T; Steffan, B; Kirsch, A; Grill, M; Gerstenberger, C; Gugatschka, M.
Exploring the Pathophysiology of Reinke's Edema: The Cellular Impact of Cigarette Smoke and Vibration.
Laryngoscope. 2021; 131(2):E547-E554
Doi: 10.1002/lary.28855
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Grill Magdalena
- Grossmann Tanja
- Co-Autor*innen der Med Uni Graz
- Gerstenberger Claus
- Gugatschka Markus
- Kirsch Andrijana
- Steffan Barbara
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- OBJECTIVES: To explore the isolated or combined effects of cigarette smoke extract (CSE) and vibration on human vocal fold fibroblasts (hVFF) in an in vitro setting in order to elucidate their influence in the pathophysiology of Reinke's edema (RE). STUDY DESIGN: Immortalized hVFF were exposed to CSE or control medium under static or vibrational conditions. A phonomimetic bioreactor was used to deliver vibrational patterns to hVFF over a period of 5 days. METHODS: Cytotoxicity was quantified using a lactate dehydrogenase assay. We employed reverse transcription-quantitative polymerase chain reaction, enzyme-linked immunosorbent assay, and Magnetic Luminex(R) assays (R&D Systems, Minneapolis, MN) to assess the influence on extracellular matrix production, fibrogenesis, inflammation, and angiogenesis. RESULTS: We observed significant changes induced by CSE alone (hyaluronic acid, matrix metalloproteinase 1, Interleukin-8, cyclooxygenase [COX]1, COX2, vascular endothelial growth factor [VEGF]D), as well as settings in which only the combination of CSE and vibration led to significant changes (transforming growth factor beta 1, VEGFA, VEGFC). Also, CSE-induced levels of COX2 were only significantly reduced when vibration was applied. CONCLUSION: We were able to explore the cellular effects of CSE and vibration on hVFF by employing a phonomimetic bioreactor. Whereas cigarette smoke is generally accepted as a risk factor for RE, the role of vibration remained unclear as it is difficult to study in humans. Our data showed that some genes and proteins in the pathophysiological context of RE were only affected when CSE in combination with vibration was applied. LEVEL OF EVIDENCE: NA Laryngoscope, 131:E547-E554, 2021.
- Find related publications in this database (using NLM MeSH Indexing)
- Cell Line - administration & dosage
- Cigarette Smoking - adverse effects
- Enzyme-Linked Immunosorbent Assay - administration & dosage
- Extracellular Matrix - drug effects, physiology
- Female - administration & dosage
- Fibroblasts - drug effects, physiology
- Humans - administration & dosage
- Inflammation - chemically induced, etiology, physiopathology
- L-Lactate Dehydrogenase - metabolism
- Laryngeal Edema - chemically induced, etiology, physiopathology
- Middle Aged - administration & dosage
- Reverse Transcriptase Polymerase Chain Reaction - administration & dosage
- Vibration - adverse effects
- Vocal Cords - cytology, drug effects, physiopathology
- Find related publications in this database (Keywords)
- Reinke's edema
- vocal fold fibroblasts
- cigarette smoke
- benign vocal fold lesions
- phonomimetic bioreactor