Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Graziani, A; Bacsa, B; Krivic, D; Wiedner, P; Curcic, S; Schindl, R; Tiapko, O; Groschner, K.
Light-Mediated Control over TRPC3-Mediated NFAT Signaling.
Cells. 2020; 9(3): 556
Doi: 10.3390/cells9030556
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- Führende Autor*innen der Med Uni Graz
- Graziani Annarita
- Groschner Klaus
- Tiapko Oleksandra
- Co-Autor*innen der Med Uni Graz
- Bacsa Bernadett
- Curcic Sanja
- Krivic Denis
- Schindl Rainer
- Wiedner Patrick
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- Abstract:
- Canonical transient receptor potential (TRPC) channels were identified as key players in maladaptive remodeling, with nuclear factor of activated T-cells (NFAT) transcription factors serving as downstream targets of TRPC-triggered Ca2+ entry in these pathological processes. Strikingly, the reconstitution of TRPC-NFAT signaling by heterologous expression yielded controversial results. Specifically, nuclear translocation of NFAT1 was found barely responsive to recombinant TRPC3, presumably based on the requirement of certain spatiotemporal signaling features. Here, we report efficient control of NFAT1 nuclear translocation in human embryonic kidney 293 (HEK293) cells by light, using a new photochromic TRPC benzimidazole activator (OptoBI-1) and a TRPC3 mutant with modified activator sensitivity. NFAT1 nuclear translocation was measured along with an all-optical protocol to record local and global Ca2+ pattern generated during light-mediated activation/deactivation cycling of TRPC3. Our results unveil the ability of wild-type TRPC3 to produce constitutive NFAT nuclear translocation. Moreover, we demonstrate that TRPC3 mutant that lacks basal activity enables spatiotemporally precise control over NFAT1 activity by photopharmacology. Our results suggest tight linkage between TRPC3 activity and NFAT1 nuclear translocation based on global cellular Ca2+ signals.
- Find related publications in this database (Keywords)
- transient receptor potential channels
- TRPC3 pharmacology
- NFAT nuclear translocation
- photochromic ligands
- OptoBI-1