Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Heger, LA; Hortmann, M; Albrecht, M; Colberg, C; Peter, K; Witsch, T; Stallmann, D; Zirlik, A; Bode, C; Duerschmied, D; Ahrens, I.
Inflammation in acute coronary syndrome: Expression of TLR2 mRNA is increased in platelets of patients with ACS.
PLoS One. 2019; 14(10): e0224181-e0224181.
Doi: 10.1371/journal.pone.0224181
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- Co-Autor*innen der Med Uni Graz
- Zirlik Andreas
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- Abstract:
- Platelets are key components in atherogenesis and determine the course of its clinical sequelae acute coronary syndrome (ACS). Components of the innate immune system-the superfamily of TLR receptors-are present in platelets and represent a link between atherothrombosis and inflammation. We hypothesize that alteration in platelet TLR mRNA expression is a result of inflammation driving coronary atherosclerosis and may represent an alternative platelet activation pathway in ACS. TLR2-, TLR4- and TLR9- mRNA-expression was determined in ACS patients and compared to patients with invasive exclusion of atherosclerotic lesions of coronary arteries. A total of fifty-four patients were enrolled in this clinical retrospective cohort single centre study. Total RNA from sepharose-filtered highly purified platelets was isolated using acid guanidinium thiocyanate-phenol-chloroform extraction and transcribed to cDNA using a first strand cDNA synthesis kit. To determine absolute copy numbers of TLR2, TLR4 and TLR9 we used plasmid based quantitative PCR with normalisation to an internal control. We found that mRNA expression levels of TLR2 but not TLR 4 and 9 are up-regulated in platelets of patients with ACS when compared to patients without coronary atherosclerosis. Our results suggest elevated TLR2 mRNA expression in platelets as a biomarker reflecting the underlying inflammation in ACS and possibly severity of coronary atherosclerosis. Platelet TLR2 may represent a link between inflammation and atherothrombosis in ACS.
- Find related publications in this database (using NLM MeSH Indexing)
- Acute Coronary Syndrome - blood
- Acute Coronary Syndrome - diagnosis
- Acute Coronary Syndrome - epidemiology
- Acute Coronary Syndrome - genetics
- Aged -
- Blood Platelets - metabolism
- Case-Control Studies -
- Coronary Vessels - metabolism
- Coronary Vessels - pathology
- Female -
- Female - epidemiology
- Humans -
- Incidence -
- Inflammation - physiopathology
- Male -
- Middle Aged -
- RNA, Messenger - genetics
- Retrospective Studies -
- Toll-Like Receptor 2 - genetics