Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bodenlenz, M; Schaupp, LA; Druml, T; Sommer, R; Wutte, A; Schaller, HC; Sinner, F; Wach, P; Pieber, TR.
Measurement of interstitial insulin in human adipose and muscle tissue under moderate hyperinsulinemia by means of direct interstitial access.
Am J Physiol Endocrinol Metab. 2005; 289(2):E296-E300
Doi: 10.1152/ajpendo.00431.2004
[OPEN ACCESS]
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Führende Autor*innen der Med Uni Graz
- Pieber Thomas
- Weiss Manfred
- Co-Autor*innen der Med Uni Graz
- Schaupp Lukas
- Sinner Frank Michael
- Wutte Andrea-Maria
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Insulin's action to stimulate glucose utilization is determined by the insulin concentration in interstitial fluid (ISF) of insulin-sensitive tissues. The concentration of interstitial insulin has been measured in human subcutaneous adipose tissue and skeletal muscle, however, never in parallel. The aim of this study was to compare interstitial insulin levels between both tissue beds by simultaneous measurements and to verify and quantify low peripheral ISF insulin fractions as found during moderate hyperinsulinemia. Nine healthy subjects (27.2 +/- 0.8 yr) were investigated. A euglycemic-hyperinsulinemic clamp was started with a primed-constant intravenous insulin infusion of 1 mU x kg(-1) x min(-1). For direct access to ISF, macroscopically perforated open-flow microperfusion catheters were inserted in both tissues. During steady-state conditions (9.5 h), interstitial effluents were collected in 30-min fractions using five different insulin concentrations in the inflowing perfusates ("no net flux" protocol). Regression analysis of insulin concentrations in perfusates and effluents yielded the relative recovery and the perfusate insulin concentration, which was in equilibrium with the surrounding tissue. Thus, in subcutaneous adipose tissue and skeletal muscle, the mean ISF-to-serum insulin level was calculated as 21.0% [95% confidence interval (CI) 17.5-24.5] and 26.0% (95% CI 19.1-32.8; P = 0.14), respectively. Recoveries for insulin averaged 51 and 64%, respectively. The data suggest that the concentrations of insulin arising in healthy subjects at the level of ISF per se are comparable between subcutaneous adipose and skeletal muscle tissue. The low interstitial insulin fractions seem to confirm reports of low peripheral insulin levels during moderate insulin clamps.
- Find related publications in this database (using NLM MeSH Indexing)
- Adipose Tissue - chemistry
- Adipose Tissue - metabolism
- Adult -
- Extracellular Fluid - chemistry
- Extracellular Fluid - metabolism
- Glucose Clamp Technique -
- Humans -
- Insulin - analysis
- Insulin - metabolism
- Muscle, Skeletal - chemistry
- Muscle, Skeletal - metabolism
- Reference Values -
- Find related publications in this database (Keywords)
- extracellular fluid
- euglycemic-hyperinsulinemic glucose clamp
- open-flow microperfusion
- no net flux
- equilibrium method