Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Ullrich, E; Heidinger, P; Soh, J; Villanova, L; Grabuschnig, S; Bachler, T; Hirschböck, E; Sánchez-Heredero, S; Ford, B; Sensen, M; Rosales, Rodriguez, I; Schwendenwein, D; Neumeister, P; Zurl, CJ; Krause, R; Lorenz, Khol, J; Sensen, CW.
Evaluation of host-based molecular markers for the early detection of human sepsis.
J Biotechnol. 2020; 310:80-88
Doi: 10.1016/j.jbiotec.2020.01.013
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- Führende Autor*innen der Med Uni Graz
- König Elisabeth
- Co-Autor*innen der Med Uni Graz
- Krause Robert
- Neumeister Peter
- Zurl Christoph Johann
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- Abstract:
- We have identified 24 molecular markers, based on circulating nucleic acids (CNA) originating from the human genome, which in combination can be used in a quantitative real-time PCR (qPCR) assay to identify the presence of human sepsis, starting two to three days before the first clinical signs develop and including patients who meet the SEPSIS-3 criteria. The accuracy was more than 87 % inside of the same patient cohort for which the markers were developed and up to 81 % in blind studies of patient cohorts which were not included in the marker development. As our markers are host-based, they can be used to capture bacterial as well as fungal sepsis, unlike the current PCR-based tests, which require species-specific primer sets for each organism causing human sepsis. Our assay directly uses an aliquot of cell-free blood as the substrate for the PCR reaction, thus allowing to obtain the diagnostic results in three to four hours after the collection of the blood samples.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent - administration & dosage
- Adult - administration & dosage
- Aged - administration & dosage
- Aged, 80 and over - administration & dosage
- Biomarkers - blood
- Cohort Studies - administration & dosage
- DNA, Bacterial - blood, genetics
- DNA, Fungal - blood, genetics
- Female - administration & dosage
- Humans - administration & dosage
- Male - administration & dosage
- Middle Aged - administration & dosage
- Real-Time Polymerase Chain Reaction - administration & dosage
- Sepsis - blood, genetics, microbiology
- Find related publications in this database (Keywords)
- Circulating nucleic acids
- Host-based markers
- Human sepsis diagnostics