Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Mai, G; Bucher, P; Morel, P; Mei, J; Bosco, D; Andres, A; Mathe, Z; Wekerle, T; Berney, T; Bühler, LH.
Anti-CD154 mAb treatment but not recipient CD154 deficiency leads to long-term survival of xenogeneic islet grafts.
Am J Transplant. 2005; 5(5):1021-1031
Doi: 10.1111/j.1600-6143.2005.00795.x
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- Co-Autor*innen der Med Uni Graz
- Mathe Zoltan
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- Abstract:
- The aim of the study was to evaluate the role of recipient CD40 and CD154 in the rejection process of concordant and discordant islets xenotransplantation (Tx). Diabetic C57BL/6 mice, CD40- or CD154-knockout (KO) and complement C3-deficient (C3-/-) mice were transplanted with either rat or human islets. Group 1, C57BL/6 Tx without therapy; Group 2, C57BL/6 Tx with anti-CD154 monoclonal antibody (mAb) (MR1) therapy; Group 3, CD40-KO; and Group 4, CD154-KO Tx without therapy; Group 5, C3-/- Tx without therapy and Group 6, C3-/-Tx with MR1 therapy. Mixed lymphocyte reactions (MLR) were performed. Compared to Group 1, MR1 induced long-term survival of xenografts in Group 2, but not in Group 6, survival of islets was not prolonged significantly in Groups 3 and 4. MLR responses in Group 2 were reduced approximately 50% compared to Group 1. In Groups 3, 4 and 6, MLR responses were not modified by the absence of CD40 or CD154 molecules, or MR1 and were similar to Group 1. Improved graft survival and reduced MLR responses in Group 2, but not in Group 6, could be explained by specific targeting of activated T cells with inactivation by complement- or cellular-mediated mechanisms. Rejection of xenografts and strong MLR responses in Groups 3 and 4 are possible through efficient activation of alternate pathways of costimulation.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antibodies, Monoclonal - chemistry
- Body Weight -
- CD40 Antigens - biosynthesis
- CD40 Antigens - genetics
- CD40 Antigens - physiology
- CD40 Ligand - genetics
- CD40 Ligand - immunology
- CD40 Ligand - physiology
- Complement C3 - genetics
- Complement C3 - physiology
- Graft Rejection -
- Graft Survival -
- Immunohistochemistry -
- Islets of Langerhans - metabolism
- Islets of Langerhans Transplantation - methods
- Lymphocyte Culture Test, Mixed -
- Lymphocytes - metabolism
- Male -
- Mice -
- Mice, Inbred C57BL -
- Mice, Knockout -
- Mice, Transgenic -
- Neoplasm Transplantation -
- Rats -
- Rats, Sprague-Dawley -
- Time Factors -
- Transplantation, Heterologous -
- Treatment Outcome -
- Find related publications in this database (Keywords)
- anti-CD154 monoclonal Ab
- CD40
- CD154
- concordant
- costimulatory blockade
- discordant
- islet xenotransplantation