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Piccioli, P; Di Luzio, A; Amann, R; Schuligoi, R; Surani, MA; Donnerer, J; Cattaneo, A.
Neuroantibodies: ectopic expression of a recombinant anti-substance P antibody in the central nervous system of transgenic mice.
Neuron. 1995; 15(2):373-384 Doi: 10.1016%2F0896-6273%2895%2990041-1 [OPEN ACCESS]
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Co-authors Med Uni Graz: Amann Rainer; Donnerer Josef; Schuligoi Rufina
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Abstract:: Recombinant antibodies are efficiently secreted by cells of the nervous system. Thus, their local expression in the CNS of transgenic mice could be used to perturb the function of the corresponding antigen. As a first application of this approach, we have generated transgenic mice that express antibodies against the neuropeptide substance P, under the transcriptional control of the promoter of the neuronal gene vgf. The transgenic antibodies are expressed in a tissue-specific and developmentally regulated manner and are effective in competing with the endogenous substance P, as demonstrated by a marked inhibition of neurogenic inflammation and by motor deficits. This phenotypic knockout approach may provide a complementary alternative to gene knockout by homologous recombination.
Find related publications in this database (using NLM MeSH Indexing): Animals -; Antibodies, Monoclonal - biosynthesis; Brain - metabolism; Escape Reaction - physiology; Gene Expression Regulation, Developmental - physiology; Inflammation - chemically induced; Mice - chemically induced; Mice, Transgenic - chemically induced; Nerve Tissue Proteins - biosynthesis; Neuropeptides - biosynthesis; Organ Specificity - biosynthesis; Promoter Regions (Genetics) - biosynthesis; Proteins - genetics; Rats - genetics; Recombinant Fusion Proteins - biosynthesis; Substance P - antagonists and inhibitors; Transcription, Genetic - antagonists and inhibitors