Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Bucher, P; Mathe, Z; Bosco, D; Andres, A; Kurfuerst, M; Rämsch-Günther, N; Buhler, L; Morel, P; Berney, T.
Serva collagenase NB1: a new enzyme preparation for human islet isolation.
Transplant Proc. 2004; 36(4):1143-1144
Doi: 10.1016/j.transproceed.2004.04.023
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Mathe Zoltan
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- Abstract:
- Advances in the rate of success of human islet isolation are due in part to the availability of new purified enzyme blends. In this study we evaluated a new enzyme preparation composed of a highly purified collagenase that can be reproducibly blended with predetermined amounts of separately packaged neutral protease. Nine human islet isolations were performed with collagenase NB1 supplemented with neutral protease (Serva Electrophoresis GMbH, group I). Yields, purity, morphology, in vitro function and islet cell apoptosis were assessed. The results were compared to those of nine human islet isolations performed with Liberase (Roche, group II) and matched for donor age, BMI, and circumstances of death. Islet yields were similar in both groups. However, islet equivalents (IE) per gram of pancreas and IE number to islet number were higher in group I (P <.05). Stimulation indices after insulin response to glucose (static incubation) were similar in both groups. Islet cell apoptosis rate was statistically significantly lower in group I. Islet morphology was significantly improved in group I with a higher proportion of intact islets. This new enzyme preparation (collagenase NB1 with neutral protease adjunct) was as effective as Liberase in terms of islet yields and function. Islet morphology was improved and rate of islet cell apoptosis was lower with this new collagenase. The absence of lot-to-lot variability in terms of neutral protease to collagenase ratio makes collagenase NB1 a promising enzyme for human islet isolation.
- Find related publications in this database (using NLM MeSH Indexing)
- Body Mass Index -
- Cell Separation - methods
- Collagenases -
- Humans -
- Islets of Langerhans - cytology
- Peptide Hydrolases -