Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Kargl, J; Zhu, X; Zhang, H; Yang, GHY; Friesen, TJ; Shipley, M; Maeda, DY; Zebala, JA; McKay-Fleisch, J; Meredith, G; Mashadi-Hossein, A; Baik, C; Pierce, RH; Redman, MW; Thompson, JC; Albelda, SM; Bolouri, H; Houghton, AM.
Neutrophil content predicts lymphocyte depletion and anti-PD1 treatment failure in NSCLC.
JCI Insight. 2019; 4(24):
Doi: 10.1172/jci.insight.130850
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- Leading authors Med Uni Graz
- Kargl Julia
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- Abstract:
- Immune checkpoint inhibitor (ICI) treatment has recently become a first-line therapy for many non-small cell lung cancer (NSCLC) patients. Unfortunately, most NSCLC patients are refractory to ICI monotherapy, and initial attempts to address this issue with secondary therapeutics have proven unsuccessful. To identify entities precluding CD8+ T cell accumulation in this process, we performed unbiased analyses on flow cytometry, gene expression, and multiplexed immunohistochemical data from a NSCLC patient cohort. The results revealed the presence of a myeloid-rich subgroup, which was devoid of CD4+ and CD8+ T cells. Of all myeloid cell types assessed, neutrophils were the most highly associated with the myeloid phenotype. Additionally, the ratio of CD8+ T cells to neutrophils (CD8/PMN) within the tumor mass optimally distinguished between active and myeloid cases. This ratio was also capable of showing the separation of patients responsive to ICI therapy from those with stable or progressive disease in 2 independent cohorts. Tumor-bearing mice treated with a combination of anti-PD1 and SX-682 (CXCR1/2 inhibitor) displayed relocation of lymphocytes from the tumor periphery into a malignant tumor, which was associated with induction of IFN-γ-responsive genes. These results suggest that neutrophil antagonism may represent a viable secondary therapeutic strategy to enhance ICI treatment outcomes.
- Find related publications in this database (using NLM MeSH Indexing)
- Aged -
- Animals -
- Antineoplastic Agents, Immunological - pharmacology
- Antineoplastic Agents, Immunological - therapeutic use
- CD8-Positive T-Lymphocytes - immunology
- Carcinoma, Non-Small-Cell Lung - blood
- Carcinoma, Non-Small-Cell Lung - drug therapy
- Carcinoma, Non-Small-Cell Lung - immunology
- Cohort Studies -
- Datasets as Topic -
- Disease Models, Animal -
- Female -
- Flow Cytometry -
- Gene Expression Profiling -
- Humans -
- Immunohistochemistry -
- Leukocyte Count -
- Lung Neoplasms - blood
- Lung Neoplasms - drug therapy
- Lung Neoplasms - immunology
- Lymphocytes, Tumor-Infiltrating - immunology
- Male -
- Mice -
- Middle Aged -
- Neutrophils - immunology
- Neutrophils - metabolism
- Programmed Cell Death 1 Receptor - antagonists & inhibitors
- Programmed Cell Death 1 Receptor - immunology
- Receptors, Interleukin-8A - antagonists & inhibitors
- Receptors, Interleukin-8B - antagonists & inhibitors
- Treatment Failure -