Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Pausan, MR; Csorba, C; Singer, G; Till, H; Schöpf, V; Santigli, E; Klug, B; Högenauer, C; Blohs, M; Moissl-Eichinger, C.
Exploring the Archaeome: Detection of Archaeal Signatures in the Human Body.
Front Microbiol. 2019; 10: 2796-2796.
Doi: 10.3389/fmicb.2019.02796
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- Führende Autor*innen der Med Uni Graz
- Moissl-Eichinger Christine
- Pausan Manuela-Raluca
- Co-Autor*innen der Med Uni Graz
- Blohs Marcus
- Hoegenauer Christoph
- Santigli Elisabeth
- Schinagl Barbara
- Singer Georg
- Till Holger
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- Abstract:
- Due to their fundamentally different biology, archaea are consistently overlooked in conventional microbiome surveys. Using amplicon sequencing, we evaluated methodological set-ups to detect archaea in samples from five different body sites: respiratory tract (nasal cavity), digestive tract (mouth, appendix, and stool) and skin. With optimized protocols, the detection of archaeal ribosomal sequence variants (RSVs) was increased from one (found in currently used, so-called "universal" approach) to 81 RSVs in a representative sample set. The results from this extensive primer-evaluation led to the identification of the primer pair combination 344f-1041R/519F-806R which performed superior for the analysis of the archaeome of gastrointestinal tract, oral cavity and skin. The proposed protocol might not only prove useful for analyzing the human archaeome in more detail but could also be used for other holobiont samples. Copyright © 2019 Pausan, Csorba, Singer, Till, Schöpf, Santigli, Klug, Högenauer, Blohs and Moissl-Eichinger.
- Find related publications in this database (Keywords)
- human archaeome
- amplicon sequencing
- human body
- detection
- methodology