Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Fröhlich, E; Öhlinger, K; Meindl, C; Corzo, C; Lochmann, D; Reyer, S; Salar-Behzadi, S.
In vitro toxicity screening of polyglycerol esters of fatty acids as excipients for pulmonary formulations.
Toxicol Appl Pharmacol. 2019; 386: 114833-114833.
Doi: 10.1016/j.taap.2019.114833
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- Co-Autor*innen der Med Uni Graz
- Fröhlich Eleonore
- Meindl Claudia
- Öhlinger Kristin Anna
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- Abstract:
- One of the main problems for the development of pulmonary formulations is the low availability of approved excipients. Polyglycerol esters of fatty acids (PGFA) are promising molecules for acting as excipient for formulation development and drug delivery to the lung. However, their biocompatibility in the deep lung has not been studied so far. Main exposed cells include alveolar epithelial cells and alveolar macrophages. Due to the poor water-solubility of PGFAs, the exposure of alveolar macrophages is expected to be much higher than that of epithelial cells. In this study, two PGFAs and their mixture were tested regarding cytotoxicity to epithelial cells and cytotoxicity and functional impairment of macrophages. Cytotoxicity was assessed by dehydrogenase activity and lactate dehydrogenase release. Lysosome function, phospholipid accumulation, phagocytosis, nitric oxide production, and cytokine release were used to evaluate macrophage function. Cytotoxicity was increased with the increased polarity of PGFA molecules. At concentrations above 1 mg/ml accumulation in lysosomes, impairment of phagocytosis, secretion of nitric oxide, and increased release of cytokines were noted. The investigated PGFAs in concentrations up to 1 mg/ml can be considered as uncritical and are promising for advanced pulmonary delivery of high powder doses and drug targeting to alveolar macrophages. Copyright © 2019 Elsevier Inc. All rights reserved.
- Find related publications in this database (Keywords)
- Polyglycerol esters of fatty acids
- Lipid-based excipients
- Alveolar macrophage
- Alveolar epithelial cells
- in vitro Lung Toxicity