Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Ondruschka, B; Woydt, L; Bernhard, M; Franke, H; Kirsten, H; Löffler, S; Pohlers, D; Hammer, N; Dreßler, J.
Post-mortem in situ stability of serum markers of cerebral damage and acute phase response.
Int J Legal Med. 2019; 133(3):871-881
Doi: 10.1007/s00414-018-1925-2
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Hammer Niels
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- Abstract:
- The aim of the given study was to test the in situ stability of biochemical markers of cerebral damage and acute phase response in the early post-mortem interval to assess their usability for forensic pathology. A monocentric, prospective study investigated post-mortem femoral venous blood samples at four time points obtained within 48 h post-mortem starting at the death of 20 deceased, using commercial immunoassays for the ten parameters: S100 calcium-binding protein B (S100B), glial fibrillary acidic protein (GFAP), neuron-specific enolase (NSE), brain-derived neurotrophic factor (BDNF), interleukin-6 (IL-6), C-reactive protein (CRP), procalcitonin (PCT), ferritin, soluble tumor necrosis factor receptor type 1 (sTNFR1), and lactate dehydrogenase (LDH). Significant changes in serum levels were observed only later than 2 h after death for all markers. Inter-laboratory comparability was high, and intra-assay precision was sufficient for most markers. Most of the biomarker levels depended on the severity of hemolysis and lipemia but were robust against freeze-thaw cycles. Serum levels increased with longer post-mortem intervals for S100B, NSE, ferritin, sTNFR1, and LDH (for all p < 0.001) but decreased over this period for CRP (p = 0.089) and PCT (p < 0.001). Largely unchanged median values were found for GFAP (p = 0.139), BDNF (p = 0.106), and IL-6 (p = 0.094). Serum levels of CRP (p = 0.059) and LDH (p = 0.109) did not differ significantly between the final ante-mortem (resuscitation) and the first post-mortem sample (moment of death). Collecting the post-mortem blood sample as soon as possible will reduce the influence of post-mortem blood changes. Serum GFAP for detection of cerebral damage as well as serum IL-6 and CRP as proof of acute phase response seemed to be preferable due to their in situ stability in the first 2 days after death.
- Find related publications in this database (using NLM MeSH Indexing)
- Acute-Phase Reaction -
- Adult -
- Aged -
- Aged, 80 and over -
- Biomarkers - blood
- Brain Injuries - blood
- Brain-Derived Neurotrophic Factor - blood
- C-Reactive Protein - analysis
- Female -
- Ferritins - blood
- Glial Fibrillary Acidic Protein - blood
- Humans -
- Immunoassay -
- Interleukin-6 - blood
- L-Lactate Dehydrogenase - blood
- Male -
- Middle Aged -
- Phosphopyruvate Hydratase - blood
- Postmortem Changes -
- Procalcitonin - blood
- Prospective Studies -
- Receptors, Tumor Necrosis Factor, Type I - blood
- S100 Calcium Binding Protein beta Subunit - blood
- Find related publications in this database (Keywords)
- Acute phase response
- Intra-individual stability
- Post-mortem biochemistry
- Serum
- Thanatochemistry
- Traumatic brain injury