Medizinische Universität Graz - Research portal

Go directly to the nagivation.
Go directly to the content.

Navigation/Search

Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Öhlinger, K; Kolesnik, T; Meindl, C; Gallé, B; Absenger-Novak, M; Kolb-Lenz, D; Fröhlich, E.
Air-liquid interface culture changes surface properties of A549 cells.
Toxicol In Vitro. 2019; 60(7): 369-382. Doi: 10.1016/j.tiv.2019.06.014
Web of Science PubMed FullText FullText_MUG

Leading authors Med Uni Graz: Fröhlich Eleonore; Öhlinger Kristin Anna
Co-authors Med Uni Graz: Absenger-Novak Markus; Gallé Birgit; Kolb Dagmar; Kolesnik Tatjana; Meindl Claudia
Altmetrics:
Dimensions Citations:
Plum Analytics:
Scite (citation analytics):
Abstract:: A549 cells are common models in the assessment of respiratory cytotoxicity. To provide physiologically more representative exposure conditions and increase the differentiation state, respiratory cells, for instance Calu-3 bronchial epithelial cells, are cultured at an air-liquid interface (ALI). There are indications that A549 cells also change their phenotype upon culture in ALI. The influence of culture in two variations of transwell cultures compared to conventional culture in plastic wells on the phenotype of A549 cells was studied. Cells were characterized by morphology, proliferation and transepithelial electrical resistance, whole genome transcription analysis, Western blot and immunocytochemical detection of pro-surfactant proteins. Furthermore, lipid staining, surface morphology, cell elasticity, surface tension and reaction to quartz particles were performed. Relatively small changes were noted in the expression of differentiation markers for alveolar cells but A549 cells cultured in ALI showed marked differences in lipid staining and surface morphology, surface tension and cytotoxicity of quartz particles. Data show that changes in physiological reactions of A549 cells in ALI culture were rather caused by change of surface properties than by increased expression of surfactant proteins. Copyright © 2019 Elsevier Ltd. All rights reserved.
Find related publications in this database (Keywords): Respiratory toxicity; Air-liquid interface; Surface morphology; 3D culture; Cytotoxicity