Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Rössler, I; Embacher, J; Pillet, B; Murat, G; Liesinger, L; Hafner, J; Unterluggauer, JJ; Birner-Gruenberger, R; Kressler, D; Pertschy, B.
Tsr4 and Nap1, two novel members of the ribosomal protein chaperOME.
Nucleic Acids Res. 2019; 47(13):6984-7002
Doi: 10.1093/nar/gkz317
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- Co-Autor*innen der Med Uni Graz
- Birner-Grünberger Ruth
- Liesinger Laura
- Unterluggauer Julia Judith
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- Abstract:
- Dedicated chaperones protect newly synthesized ribosomal proteins (r-proteins) from aggregation and accompany them on their way to assembly into nascent ribosomes. Currently, only nine of the ∼80 eukaryotic r-proteins are known to be guarded by such chaperones. In search of new dedicated r-protein chaperones, we performed a tandem-affinity purification based screen and looked for factors co-enriched with individual small subunit r-proteins. We report the identification of Nap1 and Tsr4 as direct binding partners of Rps6 and Rps2, respectively. Both factors promote the solubility of their r-protein clients in vitro. While Tsr4 is specific for Rps2, Nap1 has several interaction partners including Rps6 and two other r-proteins. Tsr4 binds co-translationally to the essential, eukaryote-specific N-terminal extension of Rps2, whereas Nap1 interacts with a large, mostly eukaryote-specific binding surface of Rps6. Mutation of the essential Tsr4 and deletion of the non-essential Nap1 both enhance the 40S synthesis defects of the corresponding r-protein mutants. Our findings highlight that the acquisition of eukaryote-specific domains in r-proteins was accompanied by the co-evolution of proteins specialized to protect these domains and emphasize the critical role of r-protein chaperones for the synthesis of eukaryotic ribosomes. © The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.